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AB323742

Human VISTA ELISA Kit

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Human VISTA ELISA Kit is a Sandwich ELISA for the measurement of Human VISTA in Human in Biofluids, Cell Culture Media samples.

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C10orf54, SISP1, VISTA, PP2135, UNQ730/PRO1412, VSIR, V-type immunoglobulin domain-containing suppressor of T-cell activation, Platelet receptor Gi24, Stress-induced secreted protein-1, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor, Sisp-1

6 Images
Sandwich ELISA - Human VISTA ELISA Kit (AB323742)
  • sELISA

Supplier Data

Sandwich ELISA - Human VISTA ELISA Kit (AB323742)

Example of human VISTA standard curve in Sample Diluent 75BS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human VISTA ELISA Kit (AB323742)
  • sELISA

Supplier Data

Sandwich ELISA - Human VISTA ELISA Kit (AB323742)

Interpolated concentration of native VISTA was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100% human plasma – EDTA. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 75BS.

Sandwich ELISA - Human VISTA ELISA Kit (AB323742)
  • sELISA

Supplier Data

Sandwich ELISA - Human VISTA ELISA Kit (AB323742)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean VISTA concentration was determined to be 916.9 pg/mL with a range of 447 – 2,212 pg/mL

Sandwich ELISA - Human VISTA ELISA Kit (AB323742)
  • sELISA

Supplier Data

Sandwich ELISA - Human VISTA ELISA Kit (AB323742)

Interpolated concentration of native VISTA was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human serum 25% and human plasma – heparin 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 75BS.

Sandwich ELISA - Human VISTA ELISA Kit (AB323742)
  • sELISA

Supplier Data

Sandwich ELISA - Human VISTA ELISA Kit (AB323742)

Example of human VISTA standard curve in Sample Diluent 75BS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human VISTA ELISA Kit (AB323742)
  • sELISA

Supplier Data

Sandwich ELISA - Human VISTA ELISA Kit (AB323742)

Interpolated concentration of native VISTA was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human plasma – citrate 100%, human plasma – EDTA 100%, and A-431 cell culture supernatant 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 75BS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.138 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human VISTA SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VISTA protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human VISTA with 6.138 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.6" } ]
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Recovery

[ { "sample": "Serum", "range": "91 - 118 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "97 - 110 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "89 - 116 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "88 - 109 %", "average": "= 97" }, { "sample": "Cell culture supernatant", "range": "90 - 118 %", "average": "= 107" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Immunoregulatory receptor which inhibits the T-cell response (PubMed : 24691993). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). May stimulate MMP14-mediated MMP2 activation (PubMed : 20666777).
See full target information VSIR
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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