Human Vitamin D Binding Protein ELISA Kit
- Recombinant
- SimpleStep
- What is this?
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(4 Publications)
Human Vitamin D Binding Protein ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Vitamin D Binding Protein with a sensitivity of 2.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Vitamin D-binding protein, DBP, VDB, Gc protein-derived macrophage activating factor, Gc-globulin, Group-specific component, Vitamin D-binding protein-macrophage activating factor, Gc-MAF, GcMAF, Gc, DBP-maf, GC
- ELISA
Supplier Data
ELISA - Human Vitamin D Binding Protein ELISA Kit (AB223586)
- sELISA
Supplier Data
Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (AB223586)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitamin D Binding Protein concentration was determined to be 632 μg/mL with a range of 460 – 866 μg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (AB223586)
Example of human Vitamin D Binding Protein standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (AB223586)
Interpolated concentrations of native Vitamin D Binding Protein in human serum and plasma samples.
The concentrations of Vitamin D Binding Protein were measured in duplicates, interpolated from the Vitamin D Binding Protein standard curves and corrected for sample dilution. All undiluted samples start at 1 : 500,000 in Sample Diluent NS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitamin D Binding Protein concentration was determined to be 427 μg /mL in neat serum, 380 μg /mL in neat plasma (citrate), 552 μg /mL in neat plasma (EDTA), and 271 μg/mL in neat plasma Heparin.
- sELISA
Supplier Data
Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (AB223586)
Interpolated concentrations of native Vitamin D Binding Protein in human milk, saliva, and urine samples.
The concentrations of Vitamin D Binding Protein were measured in duplicates, interpolated from the Vitamin D Binding Protein standard curves and corrected for sample dilution. All undiluted samples start at 1 : 500,000 in Sample Diluent NS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitamin D Binding Protein concentration was determined to be 5,280 ng /mL in neat milk, 288 ng/mL in neat saliva, and 56 ng /mL in neat urine.
- sELISA
Unknown
Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (AB223586)
Human Vitamin D Binding Protein Competitor standard curve comparison.
Standard curve comparison between Human Vitamin D Binding Protein SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
- sELISA
Lab
Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (AB223586)
Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human Vitamin D Binding Protein ELISA Kit ab223586 is a rapid single-wash 90-min Sandwich ELISA to measure Human Vitamin D Binding Protein in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, milk, saliva, serum, urine. This SimpleStep sensitivity is 2.8 pg/mL.
How the assay works
Human Vitamin D Binding Protein SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Vitamin D Binding Protein ELISA Kit ab223586 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Vitamin D Binding protein antibody [EPR20695-90] - BSA and Azide free (Capture) ab244921
- Anti-Vitamin D Binding protein antibody [EPR20695-240] - BSA and Azide free (Detector) ab244921
Vitamin D Binding Protein, also known as gc-globulin, is a 458 - amino acid, glycosylated protein encoded by the gene GC, which is in the albumin gene family. The primary function of this protein is to bind Vitamin D and its metabolites and to transport them to tissue. Vitamin D Binding protein is highly expressed and secreted into serum primarily from hepatic cells. There are three common alleles of the protein (Gc1f, Gc1s, and Gc2), and several minor polymorphisms. Different glycosylation sites are found on the isoforms of Vitamin D binding protein, a selectively deglycosylated form produced by B cell Я-galactosidase and T cell sialidase is known as Macrophage Activating Factor. MAF has been found to block angiogenic effects of several proteins on vascular endothelial cells.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (4)
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Nutrients 14: PubMed36558509
2022
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Biomedicines 10: PubMed35453583
2022
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Military Medical Research 9:4 PubMed35057868
2022
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Drug testing and analysis 12:711-719 PubMed32115881
2020
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