Human Vitamin D Binding Protein ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Vitamin D Binding Protein in Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma samples.
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- Nutrients 14:2022Assessment of Vitamin D Status in Slovenian Premenopausal and Postmenopausal Women, Using Total, Free, and Bioavailable 25-Hydroxyvitamin D (25(OH)D).Applications:Unspecified applicationReactive species:Unspecified reactive speciesVid Vičič,Andreja Kukec,Saša Kugler,Ksenija Geršak,Joško Osredkar,Ruža Pandel MikušPubMed 36558509
- Biomedicines 10:2022Urinary Biomarkers for Detection of Clinical Endometriosis or Adenomyosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei-Chun Chen,Chao-Min Cheng,Wan-Ting Liao,Ting-Chang ChangPubMed 35453583
- Military Medical Research 9:42022Potential roles of vitamin D binding protein in attenuating liver injury in sepsis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKun Xiao,Du-Chao Zhang,Ye Hu,Li-Cheng Song,Jian-Qiao Xu,Wan-Xue He,Pan Pan,Yu-Wei Wang,Li-Xin XiePubMed 35057868
- Drug testing and analysis 12:711-7192020Longitudinal studies of putative growth hormone (GH) biomarkers and hematological and steroidal parameters in relation to 2 weeks administration of human recombinant GH.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTobias Sieckmann,Hatem Elmongy,Magnus Ericsson,Hasanuzzaman Bhuiyan,Mikael Lehtihet,Lena EkströmPubMed 32115881
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
19.5 - 1250 pg/mL
- Assay time
1h 30m
- Sensitivity
= 2.8 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
Alternative names
Vitamin D-binding protein, DBP, VDB, Gc protein-derived macrophage activating factor, Gc-globulin, Group-specific component, Vitamin D-binding protein-macrophage activating factor, Gc-MAF, GcMAF, Gc, DBP-maf, GC
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Human Vitamin D Binding Protein ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Vitamin D Binding Protein in Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
19.5 - 1250 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 2.8 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 6 | mean - | SD - | C.V. 3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 4.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 95 | Range 91 - 99 % |
Sample type Milk | Average % = 102 | Range 93 - 108 % |
Sample type EDTA Plasma | Average % = 101 | Range 94 - 105 % |
Sample type Urine | Average % = 102 | Range 99 - 108 % |
Sample type Saliva | Average % = 99 | Range 86 - 107 % |
Sample type Heparin Plasma | Average % = 92 | Range 88 - 96 % |
Sample type Citrate plasma | Average % = 98 | Range 94 - 102 % |
Sample type Cell culture media | Average % = 95 | Range 95 - 95 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Vitamin D Binding Protein ELISA Kit (ab223586) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Vitamin D Binding Protein protein in cit plasma, edta plasma, hep plasma, milk, saliva, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vitamin D Binding Protein with 2.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Vitamin D Binding Protein, also known as gc-globulin, is a 458 - amino acid, glycosylated protein encoded by the gene GC, which is in the albumin gene family. The primary function of this protein is to bind Vitamin D and its metabolites and to transport them to tissue. Vitamin D Binding protein is highly expressed and secreted into serum primarily from hepatic cells. There are three common alleles of the protein (Gc1f, Gc1s, and Gc2), and several minor polymorphisms. Different glycosylation sites are found on the isoforms of Vitamin D binding protein, a selectively deglycosylated form produced by B cell ß-galactosidase and T cell sialidase is known as Macrophage Activating Factor. MAF has been found to block angiogenic effects of several proteins on vascular endothelial cells.
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6 product images
Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (ab223586)
Human Vitamin D Binding Protein Competitor standard curve comparison.
Standard curve comparison between Human Vitamin D Binding Protein SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (ab223586)
Example of human Vitamin D Binding Protein standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (ab223586)
Interpolated concentrations of native Vitamin D Binding Protein in human serum and plasma samples.
The concentrations of Vitamin D Binding Protein were measured in duplicates, interpolated from the Vitamin D Binding Protein standard curves and corrected for sample dilution. All undiluted samples start at 1:500,000 in Sample Diluent NS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitamin D Binding Protein concentration was determined to be 427 μg /mL in neat serum, 380 μg /mL in neat plasma (citrate), 552 μg /mL in neat plasma (EDTA), and 271 μg/mL in neat plasma Heparin.
Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (ab223586)
Interpolated concentrations of native Vitamin D Binding Protein in human milk, saliva, and urine samples.
The concentrations of Vitamin D Binding Protein were measured in duplicates, interpolated from the Vitamin D Binding Protein standard curves and corrected for sample dilution. All undiluted samples start at 1:500,000 in Sample Diluent NS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitamin D Binding Protein concentration was determined to be 5,280 ng /mL in neat milk, 288 ng/mL in neat saliva, and 56 ng /mL in neat urine.
Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (ab223586)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitamin D Binding Protein concentration was determined to be 632 μg/mL with a range of 460 – 866 μg/mL.
Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (ab223586)
Background-subtracted data values (mean +/- SD) are graphed.
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