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Human Vitronectin/S-Protein ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Vitronectin/S-Protein in Serum samples.

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Images

Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (AB234577), expandable thumbnail
  • Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (AB234577), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.06 - 4 ng/mL
Assay time
1h 30m
Sensitivity
= 19.9 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.

Alternative names

Vitronectin, VN, S-protein, Serum-spreading factor, V75, VTN

What's included?

1 x 96 Tests
Components
10X Human Vitronectin/S-Protein Capture Antibody
1 x 600 µL
10X Human Vitronectin/S-Protein Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
50X Cell Extraction Enhancer Solution (ab193971)
1 x 1 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent 4BI
1 x 6 mL
Human Vitronectin/S-Protein Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human Vitronectin/S-Protein ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Vitronectin/S-Protein in Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.06 - 4 ng/mL
Assay time
1h 30m
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 19.9 pg/mL

Precision

Intra assay

Sample
Recombinant Protein
n
6
mean
-
SD
-
C.V.
3

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 114.9
Range
107 - 119.2 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Human Vitronectin/S-Protein ELISA Kit (ab234577) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Vitronectin/S-Protein protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vitronectin/S-Protein with 19.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (ab234577), expandable thumbnail

    Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (ab234577)

    Example of human Vitronectin standard curve in 1X Cell Extraction Buffer PTR.

    The Vitronectin standard curve was prepared as described in Section 10.

  • Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (ab234577), expandable thumbnail

    Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (ab234577)

    Interpolated concentrations of native Vitronectin in human serum samples.

    The concentrations of Vitronectin were measured in duplicates, interpolated from the Vitronectin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitronectin concentration was determined to be 234.2 μg/mL in serum.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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