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AB223864

Human Von Willebrand Factor ELISA Kit

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(8 Publications)

Human Von Willebrand Factor ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Von Willebrand Factor with a sensitivity of 0.079 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

F8VWF, VWF, von Willebrand factor, vWF

5 Images
ELISA - Human Von Willebrand Factor ELISA Kit (AB223864)
  • ELISA

Supplier Data

ELISA - Human Von Willebrand Factor ELISA Kit (AB223864)
Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (AB223864)
  • sELISA

Supplier Data

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (AB223864)

Example of human Von Willebrand Factor standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (AB223864)
  • sELISA

Supplier Data

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (AB223864)

Representative image of Human Von Willebrand Factor ELISA Kit (ab223864)

Components shown from left to right :

- Sample diluent NS

- 10X Human Von Willebrand Factor Detector Antibody

- Human Von Willebrand Factor Lyophilised Purified Protein (2 vials)

- Human Von Willebrand Factor Capture Antibody (Lyophilised)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (AB223864)
  • sELISA

Supplier Data

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (AB223864)

Interpolated concentrations of native Von Willebrand Factor in human serum and plasma samples.

The concentrations of Von Willebrand Factor were measured in duplicates, interpolated from the Von Willebrand Factor standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 500, plasma (citrate) 1 : 500, plasma (heparin) 1 : 500, and plasma (EDTA) 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Von Willebrand Factor concentration was determined to be 4.226 μg/mL in neat serum, 2.793 μg/mL in neat plasma (citrate), 1.774 μg/mL in neat plasma (heparin), and 2.819 μg/mL in neat plasma (EDTA).

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (AB223864)
  • sELISA

Supplier Data

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (AB223864)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Von Willebrand Factor concentration was determined to be 5.280 μg/mL with a range of 2.088 – 11.35 μg/mL.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human, Rhesus monkey

Assay type

Sandwich (quantitative)

Sensitivity

= 0.079 ng/mL

Range

0.469 - 30 ng/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Von Willebrand Factor ELISA Kit ab223864 is a rapid single-wash 90-min Sandwich ELISA to measure Human Von Willebrand Factor in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 0.079 ng/mL.

How the assay works

Human Von Willebrand Factor SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Von Willebrand Factor ELISA Kit ab223864 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Von Willebrand Factor antibody [EPR20144-204] - BSA and Azide free (Capture) ab244932
- Anti-Von Willebrand Factor antibody [EPR20144-257] - BSA and Azide free (Detector) ab244932

Von Willebrand Factor is critical in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Von Willebrand Factor also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]
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Recovery

[ { "sample": "Serum", "range": "94 - 107 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "86 - 97 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": "89 - 102 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "83 - 118 %", "average": "= 98" }, { "sample": "Cell culture media", "range": "80 - 95 %", "average": "= 88" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab223864-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB223864-CMP07" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB223864-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB223864-CMP08" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB223864-CMP05" }, { "size":"2 x 10 Vial", "name":"Human Von Willabrand Factor Lyophilized Recombinant Protein", "number":"AB223864-CMP04" }, { "size":"10 x 1 Vial", "name":"Human Von Willabrand Factor Capture Antibody (Lyophilized)", "number":"AB223864-CMP02" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB223864-CMP01" }, { "size":"1 x 6000 µL", "name":"10X Human Von Willabrand Factor Detector Antibody", "number":"AB223864-CMP03" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB223864-CMP09" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB223864-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab223864-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB223864-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB223864-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB223864-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB223864-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB223864-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human Von Willabrand Factor Capture Antibody (Lyophilized)", "number":"AB223864-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Von Willabrand Factor Lyophilized Recombinant Protein", "number":"AB223864-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB223864-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Von Willabrand Factor Detector Antibody", "number":"AB223864-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB223864-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

The protein expressed by the gene VWF is important for maintaining hemostasis. It promotes platelet adhesion at sites of vascular injury by forming a molecular bridge between the sub-endothelial collagen matrix and the platelet-surface receptor complex GPIb-IX-V. Additionally, it acts as a chaperone for coagulation factor VIII, delivering it to the injury site, stabilizing its heterodimeric structure, and protecting it from premature clearance from plasma. This supplementary information is collated from multiple sources and compiled automatically.
See full target information VWF
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10754 PubMed39737992

2024

SARS-CoV-2 infection of human pluripotent stem cell-derived vascular cells reveals smooth muscle cells as key mediators of vascular pathology during infection.

Applications

Unspecified application

Species

Unspecified reactive species

Alexsia Richards,Andrew S Khalil,Max Friesen,Troy W Whitfield,Xinlei Gao,Tenzin Lungjangwa,Roger D Kamm,Zhengpeng Wan,Lee Gehrke,David Mooney,Rudolf Jaenisch

Nature communications 14:1638 PubMed37015925

2023

Multiplatform analyses reveal distinct drivers of systemic pathogenesis in adult versus pediatric severe acute COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Druzak,Elizabeth Iffrig,Blaine R Roberts,Tiantian Zhang,Kirby S Fibben,Yumiko Sakurai,Hans P Verkerke,Christina A Rostad,Ann Chahroudi,Frank Schneider,Andrew Kam Ho Wong,Anne M Roberts,Joshua D Chandler,Susan O Kim,Mario Mosunjac,Marina Mosunjac,Rachel Geller,Igor Albizua,Sean R Stowell,Connie M Arthur,Evan J Anderson,Anna A Ivanova,Jun Ahn,Xueyun Liu,Kristal Maner-Smith,Thomas Bowen,Mirko Paiardini,Steve E Bosinger,John D Roback,Deanna A Kulpa,Guido Silvestri,Wilbur A Lam,Eric A Ortlund,Cheryl L Maier

International journal of hematology 116:937-946 PubMed35994163

2022

Plasma biomarkers associated with survival and thrombosis in hospitalized COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

David Cabrera-Garcia,Andrea Miltiades,Peter Yim,Samantha Parsons,Katerina Elisman,Mohammad Taghi Mansouri,Gebhard Wagener,Neil L Harrison

Frontiers in immunology 13:859387 PubMed35634344

2022

The Genetic Risk for COVID-19 Severity Is Associated With Defective Immune Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Yunus Kuijpers,Xiaojing Chu,Martin Jaeger,Simone J C F M Moorlag,Valerie A C M Koeken,Bowen Zhang,Aline de Nooijer,Inge Grondman,Manoj Kumar Gupta,Nico Janssen,Vera P Mourits,L Charlotte J de Bree,Quirijn de Mast,Frank L van de Veerdonk,Leo A B Joosten,Yang Li,Mihai G Netea,Cheng-Jian Xu

Anesthesiology 137:67-78 PubMed35412597

2022

Suppression of Fibrinolysis and Hypercoagulability, Severity of Hypoxemia, and Mortality in COVID-19 Patients: A Retrospective Cohort Study.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin M Corey,Lyra B Olson,Ibtehaj A Naqvi,Sarah A Morrison,Connor Davis,Shahid M Nimjee,Loretta G Que,Robin E Bachelder,Bryan D Kraft,Lingye Chen,Smita K Nair,Jerrold H Levy,Bruce A Sullenger

JACC. Basic to translational science 7:247-261 PubMed35411318

2022

Hyperadhesive von Willebrand Factor Promotes Extracellular Vesicle-Induced Angiogenesis: Implication for LVAD-Induced Bleeding.

Applications

Unspecified application

Species

Unspecified reactive species

Mengchen Yang,Katie L Houck,Xinlong Dong,Maria Hernandez,Yi Wang,Sriram S Nathan,Xiaoping Wu,Vahid Afshar-Kharghan,Xiaoyun Fu,Miguel A Cruz,Jianning Zhang,Angelo Nascimbene,Jing-Fei Dong

European journal of heart failure 23:1916-1926 PubMed34415085

2021

Association of COVID-19 with impaired endothelial glycocalyx, vascular function and myocardial deformation 4 months after infection.

Applications

Unspecified application

Species

Unspecified reactive species

Vaia Lambadiari,Asimina Mitrakou,Aikaterini Kountouri,John Thymis,Konstantinos Katogiannis,Emmanouil Korakas,Charalampos Varlamos,Ioanna Andreadou,Maria Tsoumani,Helen Triantafyllidi,Aristotelis Bamias,Konstantinos Thomas,Pinelopi Kazakou,Sotiria Grigoropoulou,Dimitra Kavatha,Anastasia Antoniadou,Meletios-Athanasios Dimopoulos,Ignatios Ikonomidis

Diagnostics (Basel, Switzerland) 11: PubMed34206615

2021

A Novel Combination of Blood Biomarkers and Clinical Stroke Scales Facilitates Detection of Large Vessel Occlusion Ischemic Strokes.

Applications

Unspecified application

Species

Unspecified reactive species

Edoardo Gaude,Barbara Nogueira,Marcos Ladreda Mochales,Sheila Graham,Sarah Smith,Lisa Shaw,Sara Graziadio,Gonzalo Ladreda Mochales,Philip Sloan,Joshua D Bernstock,Shashank Shekhar,Toby I Gropen,Christopher I Price
View all publications
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