Human Von Willebrand Factor ELISA Kit ab223864 is a single-wash 90-min SimpleStep ELISA used to quantify Human Von Willebrand Factor with a sensitivity of 0.079 ng/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human Von Willebrand Factor sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
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- Nature communications 14:16382023Multiplatform analyses reveal distinct drivers of systemic pathogenesis in adult versus pediatric severe acute COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamuel Druzak,Elizabeth Iffrig,Blaine R Roberts,Tiantian Zhang,Kirby S Fibben,Yumiko Sakurai,Hans P Verkerke,Christina A Rostad,Ann Chahroudi,Frank Schneider,Andrew Kam Ho Wong,Anne M Roberts,Joshua D Chandler,Susan O Kim,Mario Mosunjac,Marina Mosunjac,Rachel Geller,Igor Albizua,Sean R Stowell,Connie M Arthur,Evan J Anderson,Anna A Ivanova,Jun Ahn,Xueyun Liu,Kristal Maner-Smith,Thomas Bowen,Mirko Paiardini,Steve E Bosinger,John D Roback,Deanna A Kulpa,Guido Silvestri,Wilbur A Lam,Eric A Ortlund,Cheryl L MaierPubMed 37015925
- International journal of hematology 116:937-9462022Plasma biomarkers associated with survival and thrombosis in hospitalized COVID-19 patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Cabrera-Garcia,Andrea Miltiades,Peter Yim,Samantha Parsons,Katerina Elisman,Mohammad Taghi Mansouri,Gebhard Wagener,Neil L HarrisonPubMed 35994163
- Frontiers in immunology 13:8593872022The Genetic Risk for COVID-19 Severity Is Associated With Defective Immune Responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunus Kuijpers,Xiaojing Chu,Martin Jaeger,Simone J C F M Moorlag,Valerie A C M Koeken,Bowen Zhang,Aline de Nooijer,Inge Grondman,Manoj Kumar Gupta,Nico Janssen,Vera P Mourits,L Charlotte J de Bree,Quirijn de Mast,Frank L van de Veerdonk,Leo A B Joosten,Yang Li,Mihai G Netea,Cheng-Jian XuPubMed 35634344
- Anesthesiology 137:67-782022Suppression of Fibrinolysis and Hypercoagulability, Severity of Hypoxemia, and Mortality in COVID-19 Patients: A Retrospective Cohort Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristin M Corey,Lyra B Olson,Ibtehaj A Naqvi,Sarah A Morrison,Connor Davis,Shahid M Nimjee,Loretta G Que,Robin E Bachelder,Bryan D Kraft,Lingye Chen,Smita K Nair,Jerrold H Levy,Bruce A SullengerPubMed 35412597
- JACC. Basic to translational science 7:247-2612022Hyperadhesive von Willebrand Factor Promotes Extracellular Vesicle-Induced Angiogenesis: Implication for LVAD-Induced Bleeding.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengchen Yang,Katie L Houck,Xinlong Dong,Maria Hernandez,Yi Wang,Sriram S Nathan,Xiaoping Wu,Vahid Afshar-Kharghan,Xiaoyun Fu,Miguel A Cruz,Jianning Zhang,Angelo Nascimbene,Jing-Fei DongPubMed 35411318
- European journal of heart failure 23:1916-19262021Association of COVID-19 with impaired endothelial glycocalyx, vascular function and myocardial deformation 4 months after infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVaia Lambadiari,Asimina Mitrakou,Aikaterini Kountouri,John Thymis,Konstantinos Katogiannis,Emmanouil Korakas,Charalampos Varlamos,Ioanna Andreadou,Maria Tsoumani,Helen Triantafyllidi,Aristotelis Bamias,Konstantinos Thomas,Pinelopi Kazakou,Sotiria Grigoropoulou,Dimitra Kavatha,Anastasia Antoniadou,Meletios-Athanasios Dimopoulos,Ignatios IkonomidisPubMed 34415085
- Diagnostics (Basel, Switzerland) 11:2021A Novel Combination of Blood Biomarkers and Clinical Stroke Scales Facilitates Detection of Large Vessel Occlusion Ischemic Strokes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdoardo Gaude,Barbara Nogueira,Marcos Ladreda Mochales,Sheila Graham,Sarah Smith,Lisa Shaw,Sara Graziadio,Gonzalo Ladreda Mochales,Philip Sloan,Joshua D Bernstock,Shashank Shekhar,Toby I Gropen,Christopher I PricePubMed 34206615
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human, Rhesus monkey
- Range
- 0.469 - 30 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.079 ng/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Alternative names
F8VWF, VWF, von Willebrand factor, vWF
What's included?
Recommended products
Human Von Willebrand Factor ELISA Kit ab223864 is a single-wash 90-min SimpleStep ELISA used to quantify Human Von Willebrand Factor with a sensitivity of 0.079 ng/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human Von Willebrand Factor sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human, Rhesus monkey
- Range
- 0.469 - 30 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 0.079 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 6.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 101 | Range 94 - 107 % |
Sample type EDTA Plasma | Average % = 92 | Range 86 - 97 % |
Sample type Heparin Plasma | Average % = 95 | Range 89 - 102 % |
Sample type Citrate plasma | Average % = 98 | Range 83 - 118 % |
Sample type Cell culture media | Average % = 88 | Range 80 - 95 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Von Willebrand Factor ELISA Kit (ab223864) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Von Willebrand Factor protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Von Willebrand Factor with 0.079 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Von Willebrand Factor is critical in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Von Willebrand Factor also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (ab223864)
Example of human Von Willebrand Factor standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (ab223864)
Interpolated concentrations of native Von Willebrand Factor in human serum and plasma samples.
The concentrations of Von Willebrand Factor were measured in duplicates, interpolated from the Von Willebrand Factor standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:500, plasma (citrate) 1:500, plasma (heparin) 1:500, and plasma (EDTA) 1:500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Von Willebrand Factor concentration was determined to be 4.226 μg/mL in neat serum, 2.793 μg/mL in neat plasma (citrate), 1.774 μg/mL in neat plasma (heparin), and 2.819 μg/mL in neat plasma (EDTA).
Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (ab223864)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Von Willebrand Factor concentration was determined to be 5.280 μg/mL with a range of 2.088 – 11.35 μg/mL.
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