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AB326430

Human Von Willebrand Factor ELISA Kit, Chemiluminescent

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Human Von Willebrand Factor ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Von Willebrand Factor with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 27.43 - 60000 pg/ml with a sensitivity of 32.46pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

F8VWF, VWF, von Willebrand factor, vWF

5 Images
Sandwich ELISA - Human Von Willebrand Factor ELISA Kit, Chemiluminescent (AB326430)
  • sELISA

Lab

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit, Chemiluminescent (AB326430)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit, Chemiluminescent (AB326430)
  • sELISA

Lab

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit, Chemiluminescent (AB326430)

Interpolated concentrations of native Von Willebrand Factor in human serum and plasma samples.

The concentrations of Von Willebrand Factor were measured in duplicates interpolated from the Von Willebrand Factor standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 500 plasma (citrate) 1 : 500 plasma (heparin) 1 : 500 and plasma (EDTA) 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Von Willebrand Factor concentration was determined to be 4.226 µg/mL in neat serum 2.793 µg/mL in neat plasma (citrate) 1.774 µg/mL in neat plasma (heparin) and 2.819 µg/mL in neat plasma (EDTA).

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit, Chemiluminescent (AB326430)
  • sELISA

Lab

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit, Chemiluminescent (AB326430)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Von Willebrand Factor concentration was determined to be 5.280 µg/mL with a range of 2.088 – 11.35 µg/mL.

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit, Chemiluminescent (AB326430)
  • sELISA

Lab

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit, Chemiluminescent (AB326430)

Standard curve comparison between the Chemiluminescent Human Von Willebrand Factor SimpleStep ELISA and colorimetric Human Von Willebrand Factor SimpleStep ELISA (ab223864) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit, Chemiluminescent (AB326430)
  • sELISA

Lab

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit, Chemiluminescent (AB326430)

Dynamic range comparison between the Chemiluminescent Human Von Willebrand Factor SimpleStep ELISA, colorimetric Human Von Willebrand Factor SimpleStep ELISA (ab223864), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 32.46 pg/mL

Range

27.43 - 60000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Von Willebrand Factor ELISA Kit, Chemiluminescent (ab326430) is a rapid single-wash sandwich 55-min ELISA to measure Human Von Willebrand Factor in Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media with a sensitivity of 32.46 pg/mL.

How the assay works

Human Von Willebrand Factor ELISA Kit, Chemiluminescent (ab326430) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Von Willebrand Factor (ab326430) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Von Willebrand Factor antibody [EPR20144-204] - BSA and Aside free (Capture) ab244667
- Anti-Von Willebrand Factor antibody [EPR20144-218] - BSA and Aside free (Detector) ab244867
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.4" } ]
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Recovery

[ { "sample": "Serum", "range": "94 - 107 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "86 - 97 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": "89 - 102 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "83 - 118 %", "average": "= 98" }, { "sample": "Cell culture media", "range": "80 - 95 %", "average": "= 88" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
See full target information VWF
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com