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AB168548

Human Von Willebrand Factor ELISA Kit (with plasma controls)

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(3 Publications)

Human Von Willebrand Factor ELISA Kit (with plasma controls) is a Sandwich ELISA for the measurement of Human Von Willebrand Factor (with plasma controls) in Human in Biofluids samples.

View Alternative Names

F8VWF, VWF, von Willebrand factor, vWF

1 Images
Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (with plasma controls) (AB168548)
  • sELISA

Supplier Data

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (with plasma controls) (AB168548)

Representative Standard Curve using ab168548

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

> 0.07 mIU/mL

Range

0.625 - 40 mIU/mL

Assay time

5h

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Primary Antibodies

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Von Willebrand Factor (VWF) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Von Willebrand Factor levels in cell culture supernatants, plasma and serum.

A Von Willebrand Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Von Willebrand Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Von Willebrand Factor captured in plate.

Get higher sensitivity in only 90 minutes with Human Von Willebrand Factor ELISA Kit (ab189571) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab168548-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB168548-CMP03", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB168548-CMP02", "productcode":"" }, { "size":"1 x 60 µL", "name":"100X Biotinylated Human Von Williebrand Factor Antibody", "number":"AB168548-CMP10", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB168548-CMP05", "productcode":"" }, { "size":"1 x 96 Test", "name":"Von Williebrand Factor Microplate (12 x 8 well strips)", "number":"AB168548-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Low control (Depleted Human Plasma)", "number":"AB168548-CMP11", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB168548-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB168548-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive control (Reference Plasma Control)", "number":"AB168548-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Von Williebrand Factor Standard", "number":"AB168548-CMP09", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB168548-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
See full target information VWF
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 8:817305 PubMed35087853

2022

The Impact of Age and BMI on the VWF/ADAMTS13 Axis and Simultaneous Thrombin and Plasmin Generation in Hospitalized COVID-19 Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Kiruphagaran Thangaraju,Upendra Katneni,Imo J Akpan,Kenichi Tanaka,Tiffany Thomas,Saini Setua,Julie A Reisz,Francesca Cendali,Fabia Gamboni,Travis Nemkov,Stacie Kahn,Alexander Z Wei,Jacob E Valk,Krystalyn E Hudson,David J Roh,Chiara Moriconi,James C Zimring,Angelo D'Alessandro,Steven L Spitalnik,Richard O Francis,Paul W Buehler

Cells 10: PubMed34571942

2021

Biological and Clinical Factors Contributing to the Metabolic Heterogeneity of Hospitalized Patients with and without COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Angelo D'Alessandro,Tiffany Thomas,Imo J Akpan,Julie A Reisz,Francesca I Cendali,Fabia Gamboni,Travis Nemkov,Kiruphagaran Thangaraju,Upendra Katneni,Kenichi Tanaka,Stacie Kahn,Alexander Z Wei,Jacob E Valk,Krystalyn E Hudson,David Roh,Chiara Moriconi,James C Zimring,Eldad A Hod,Steven L Spitalnik,Paul W Buehler,Richard O Francis

Research square : PubMed34013258

2021

Biological and Clinical Factors contributing to the Metabolic Heterogeneity of Hospitalized Patients with and without COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Angelo D'Alessandro,Imo Akpan,Tiffany Thomas,Julie Reisz,Francesca Cendali,Fabia Gamboni,Travis Nemkov,Kiruphagaran Thangaraju,Upendra Katneni,Kenichi Tanaka,Stacie Kahn,Alexander Wei,Jacob Valk,Krystalyn Hudson,David Roh,Chiara Moriconi,James Zimring,Eldad Hod,Steven Spitalnik,Paul Buehler,Richard Francis
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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