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Human VSIG4 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human VSIG4 in Human in Serum, Plasma, Cell culture supernatant samples.

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Images

Sandwich ELISA - Human VSIG4 ELISA Kit (AB314819), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Serum, Plasma, Cell culture supernatant
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
4.9 - 1200 pg/mL
Sensitivity
= 4.9 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.

Alternative names

CRIg, Z39IG, UNQ317/PRO362, VSIG4, V-set and immunoglobulin domain-containing protein 4, Protein Z39Ig

What's included?

1 x 96 Tests
Components
200X HRP-Streptavidin
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent (Item E2)
1 x 15 mL
Biotinylated Human VSIG4 Detection Antibody
2 x 1 Vial
Human VSIG4 Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human VSIG4 Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human VSIG4 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human VSIG4 in Human in Serum, Plasma, Cell culture supernatant samples.

Key facts

Detection method
Colorimetric
Sample types
Serum, Plasma, Cell culture supernatant
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
4.9 - 1200 pg/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 4.9 pg/mL

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 105.3
Range
101 - 110 %
Sample type
Plasma
Average %
= 113.2
Range
103 - 125 %
Sample type
Cell culture media
Average %
= 123
Range
115 - 133 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Human VSIG4 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human VSIG4 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human VSIG4 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Azurocidin, BLMH, C1q R1, C1qTNF1, C2, Cathepsin A, Cathepsin D, Cathepsin X/Z/P, Aminopeptidase N, CD35, Contactin-4, CPA1, CPM, DNER, Endorepellin, Fetuin B, Hepassocin, LEKTI, MAGP-2/MFAP5, MCAM, MMR, Neuropilin-1, PAM, Park7, PCSK9, PLUNC, PRCP, PRTN3, P-Selectin, PSP94, S100A6, SAA, SLPI, SPARCL1, Tenascin C, Thioredoxin-1, TIM-4, Trypsin Pan, Thrombospondin-4.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human VSIG4 ELISA Kit (ab314819), expandable thumbnail

    Sandwich ELISA - Human VSIG4 ELISA Kit (ab314819)

    Example of standard curve using ab314819. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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