Human VTA1 ELISA Kit

Product details

The Human VTA1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222878) is designed for detection of human VTA1 in plasma, serum, and cell culture samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human VTA1 in less than 4 hours. A polyclonal antibody specific for human VTA1 has been pre-coated onto a 96-well microplate with removable strips. VTA1 in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for VTA1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Vacuolar protein sorting-associated protein VTA1 homolog (VTA1), also known as dopamine-responsive gene 1 protein (DRG-1), LYST-interacting protein 5 (LIP5), and SKD1-binding protein 1 (SBP1), is predominantly a cytosolic protein. VTA1 consists of 307 amino acids with a predicted molecular mass of 34 kDa. It is involved in the endosomal multivesicular body (MVB) formation and trafficking. The MVB is an endosomal compartment that serves to sort membrane proteins destined for degradation or routing to the lysosome. These membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids, are internalized into intraluminal vesicles that are generated by the invagination and scission from the limiting membrane of the endosome. The contents of the MVB are then transferred to lysosomes. VTA1 interacts with charged multivesicular body protein 5 (CHMP5) to form stable complexes in the cytoplasm, binds well to CHMP1B, CHMP2A, and CHMP3 proteins for MVB sorting, and is required for immunodeficiency virus type 1 HIV particle release.