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AB267596

Human WIF-1 ELISA Kit

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Human WIF-1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human WIF-1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

UNQ191/PRO217, WIF1, Wnt inhibitory factor 1, WIF-1

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Sandwich ELISA - Human WIF-1 ELISA Kit (AB267596)
  • sELISA

Supplier Data

Sandwich ELISA - Human WIF-1 ELISA Kit (AB267596)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.1 ng/mL

Range

0.102 - 25 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human WIF-1 ELISA Kit is designed for the quantitative determination of WIF-1 in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human WIF-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and WIF-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human WIF-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of WIF-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Human 2B4, ADAM-9, APRIL, BMP-2, BMP-9, Cathepsin L, FABP2, FAP, FGF-19, Galectin-3, HGFR, IFN-alpha/beta R2, IGF-2R, IL-1R6, IL-24, IL-33, Kallikrein-14, Legumain, LOX-1, MBL, Neprilysin, Notch, NOV, Osteoactivin, PD-1, PGRPs, SERPINA4, sFRP-3, Thrombomodulin, TLR2, Transferrin.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "72 - 107 %", "average": "= 86.25" }, { "sample": "Plasma", "range": "82 - 115 %", "average": "= 94.4" }, { "sample": "Cell culture media", "range": "108 - 143 %", "average": "= 133.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267596-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267596-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267596-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human WIF-1 coated Microplate (12 x 8 wells)", "number":"AB267596-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human WIF-1 Standard (Lyophilized)", "number":"AB267596-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267596-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human WIF-1 Detection Antibody", "number":"AB267596-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB267596-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267596-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB267596-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WIF1 also known as Wnt inhibitory factor 1 encodes a protein that interacts with Wnt proteins and inhibits their activity. This protein has an approximate molecular mass of 40 kDa and is secreted into the extracellular space. It shows expression in various tissues including the brain lung and kidney. The structure of WIF1 enables it to bind directly to Wnt proteins modulating their signaling capabilities.
Biological function summary

WIF1 participates in the regulation of Wnt signaling which is a critical pathway for cell development and differentiation. It functions to maintain cellular homeostasis by preventing excessive Wnt signaling an essential process in embryonic development and adult tissue maintenance. WIF1 does not form a complex but interacts with Wnt proteins in the extracellular environment ensuring that Wnt signaling remains controlled.

Pathways

WIF1 plays a significant role in the Wnt signaling pathway which is essential for processes like cell fate determination and proliferation. By inhibiting Wnt activity WIF1 supports maintaining balanced signaling levels within the pathway. It connects to other components like β-catenin and can influence pathways like the Wnt/β-catenin signaling pathway which involves the accumulation and transcriptional activity of β-catenin in the cell nucleus.

WIF1 has been implicated in cancer and osteoarthritis due to its regulatory effect on Wnt signaling. Downregulation of WIF1 often associates with increased Wnt activity contributing to tumor progression in various cancers through interactions with proteins like DKK1 a known Wnt pathway inhibitor. In osteoarthritis altered WIF1 expression levels can affect cartilage degradation by modifying Wnt signaling showing its role in maintaining joint health.
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Product protocols

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Target data

Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
See full target information WIF1
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websiteProtocolBooklet
en
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