Human WIF-1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human WIF-1 in Human in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.102 - 25 ng/mL
- Sensitivity
- = 0.1 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
Alternative names
UNQ191/PRO217, WIF1, Wnt inhibitory factor 1, WIF-1
What's included?
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Human WIF-1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human WIF-1 in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.102 - 25 ng/mL
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 0.1 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 86.25 | Range 72 - 107 % |
Sample type Plasma | Average % = 94.4 | Range 82 - 115 % |
Sample type Cell culture media | Average % = 133.9 | Range 108 - 143 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human WIF-1 ELISA Kit is designed for the quantitative determination of WIF-1 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human WIF-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and WIF-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human WIF-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of WIF-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Human 2B4, ADAM-9, APRIL, BMP-2, BMP-9, Cathepsin L, FABP2, FAP, FGF-19, Galectin-3, HGFR, IFN-alpha/beta R2, IGF-2R, IL-1R6, IL-24, IL-33, Kallikrein-14, Legumain, LOX-1, MBL, Neprilysin, Notch, NOV, Osteoactivin, PD-1, PGRPs, SERPINA4, sFRP-3, Thrombomodulin, TLR2, Transferrin.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WIF1 also known as Wnt inhibitory factor 1 encodes a protein that interacts with Wnt proteins and inhibits their activity. This protein has an approximate molecular mass of 40 kDa and is secreted into the extracellular space. It shows expression in various tissues including the brain lung and kidney. The structure of WIF1 enables it to bind directly to Wnt proteins modulating their signaling capabilities.
- Biological function summary
WIF1 participates in the regulation of Wnt signaling which is a critical pathway for cell development and differentiation. It functions to maintain cellular homeostasis by preventing excessive Wnt signaling an essential process in embryonic development and adult tissue maintenance. WIF1 does not form a complex but interacts with Wnt proteins in the extracellular environment ensuring that Wnt signaling remains controlled.
- Pathways
WIF1 plays a significant role in the Wnt signaling pathway which is essential for processes like cell fate determination and proliferation. By inhibiting Wnt activity WIF1 supports maintaining balanced signaling levels within the pathway. It connects to other components like β-catenin and can influence pathways like the Wnt/β-catenin signaling pathway which involves the accumulation and transcriptional activity of β-catenin in the cell nucleus.
- Associated diseases and disorders
WIF1 has been implicated in cancer and osteoarthritis due to its regulatory effect on Wnt signaling. Downregulation of WIF1 often associates with increased Wnt activity contributing to tumor progression in various cancers through interactions with proteins like DKK1 a known Wnt pathway inhibitor. In osteoarthritis altered WIF1 expression levels can affect cartilage degradation by modifying Wnt signaling showing its role in maintaining joint health.
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1 product image
Sandwich ELISA - Human WIF-1 ELISA Kit (ab267596)
This standard curve is for demonstration only. A standard curve must be run with each assay.
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Product protocols
- Visit the general protocols
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