JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB155445

Human WISP-1 ELISA Kit (CCN4)

Be the first to review this product! Submit a review

|

(2 Publications)

Human WISP-1 ELISA Kit (CCN4) is a Sandwich (quantitative) ELISA for the measurement of Human WISP-1 (CCN4) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

WISP1, CCN4, CCN family member 4, WNT1-inducible-signaling pathway protein 1, Wnt-1-induced secreted protein, WISP-1

2 Images
Sandwich ELISA - Human WISP-1 ELISA Kit (CCN4) (AB155445)
  • sELISA

Unknown

Sandwich ELISA - Human WISP-1 ELISA Kit (CCN4) (AB155445)

Representative standard curve using ab155445 - Assay Diluent C.

Sandwich ELISA - Human WISP-1 ELISA Kit (CCN4) (AB155445)
  • sELISA

Unknown

Sandwich ELISA - Human WISP-1 ELISA Kit (CCN4) (AB155445)

Representative standard curve using ab155445 - Assay Diluent B.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 50 pg/mL

Range

0.061 - 15 ng/mL

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's WISP-1 (CCN4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human WISP-1 in serum, plasma (collect plasma using EDTA as an anticoagulant. Heparin and Citrate are not recommended as anticoagulants for use in this assay) and cell culture supernatants.

This assay employs an antibody specific for Human WISP-1coated on a 96-well plate. Standards and samples are pipetted into the wells and WISP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human WISP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of WISP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "87 - 101 %", "average": "= 93.12" }, { "sample": "Serum", "range": "80 - 97 %", "average": "= 90.42" }, { "sample": "Plasma", "range": "119 - 135 %", "average": "= 127.1" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155445-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155445-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155445-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB155445-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"WISP-1 Microplate (12 x 8 wells)", "number":"AB155445-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human WISP-1", "number":"AB155445-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB155445-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155445-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155445-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human WISP-1 Standard (lyophilized)", "number":"AB155445-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WISP1 also known as Wnt1 Inducible Signaling Pathway Protein 1 or CCN4 is a protein with significant roles in cellular processes. This protein has an approximate mass of 41 kDa. You can find its expression in various tissues including bone cartilage and vascular tissues. WISP1 belongs to the CCN family of proteins which are matricellular proteins that interact with different cell surface molecules and receptor systems to modulate cellular functions.
Biological function summary

WISP1 plays an important role in cellular proliferation differentiation and survival. It functions as a matricellular protein that can form complexes with integrins and other cell surface receptors. This interaction influences cellular responses to environmental signals. WISP1 contributes to extracellular matrix interaction and cellular adhesion. By regulating these processes WISP1 supports tissue development repair and regeneration.

Pathways

The network of interactions influenced by WISP1 impacts several key signaling pathways including the Wnt signaling pathway and the TGF-beta pathway. These pathways are important for regulating cell growth and differentiation. In connection with the Wnt pathway WISP1 interacts with proteins like beta-catenin which plays an important role in signal transduction and gene expression. These interactions help maintain proper cellular function and structural integrity.

WISP1 shows links to cancer and fibrosis. Dysregulation of WISP1 expression and its related pathways can contribute to the development and progression of these conditions. In cancers WISP1 interacts with proteins like beta-catenin and contributes to the regulation of oncogenic processes such as tumor growth and metastasis. In fibrosis WISP1 influences the deposition of extracellular matrix proteins affecting tissue scarring and organ function.
style
left-column

Product protocols

style
left-column

Target data

Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan.
See full target information CCN4
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:11050-11060 PubMed35481425

2022

Ultrasound microbubble-mediated RNA interference targeting WNT1 inducible signaling pathway protein 1(WISP1) suppresses the proliferation and metastasis of breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Faying Fang,Weizhi Xu,Jian Zhang,Jin Gu,Gaoyi Yang

The Journal of clinical investigation 127:3510-3520 PubMed28783045

2017

Macrophage-derived IL-10 mediates mucosal repair by epithelial WISP-1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel Quiros,Hikaru Nishio,Philipp A Neumann,Dorothee Siuda,Jennifer C Brazil,Veronica Azcutia,Roland Hilgarth,Monique N O'Leary,Vicky Garcia-Hernandez,Giovanna Leoni,Mingli Feng,Gabriela Bernal,Holly Williams,Priya H Dedhia,Christian Gerner-Smidt,Jason Spence,Charles A Parkos,Timothy L Denning,Asma Nusrat
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB178547

Anti-WISP1 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (AB178547)

  • 4
  • 2
Assay Kits

AB184857

Human GLP1 (7-36) ELISA Kit

Recombinant|SimpleStep

ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

  • 5
  • 3
Assay Kits

AB260064

Human C-Peptide ELISA Kit

Recombinant|SimpleStep

ELISA - Human C-Peptide ELISA Kit (AB260064)

  • 0
  • 0
Assay Kits

AB193710

Human NOV ELISA Kit (Nephroblastoma Overexpressed)

Sandwich ELISA - Human NOV ELISA Kit (Nephroblastoma Overexpressed) (AB193710)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com