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AB314820

Human Wnt4 ELISA Kit

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Human Wnt4 ELISA Kit is a Sandwich ELISA for the measurement of Human Wnt4 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

UNQ426/PRO864, WNT4, Protein Wnt-4

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Sandwich ELISA - Human Wnt4 ELISA Kit (AB314820)
  • sELISA

Supplier Data

Sandwich ELISA - Human Wnt4 ELISA Kit (AB314820)

Example of standard curve using ab314820. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.22 ng/mL

Range

1.22 - 300 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB150596

Anti-Wnt4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt4 antibody (AB150596)

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Proteins & Peptides

AB236179

Recombinant Human Wnt4 protein (Tagged)

SDS-PAGE - Recombinant Human Wnt4 protein (Tagged) (AB236179)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Wnt4 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Wnt4 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Wnt4 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "100 - 120 %", "average": "= 107.3" }, { "sample": "Plasma", "range": "69 - 110 %", "average": "= 91.46" }, { "sample": "Cell culture media", "range": "68 - 84 %", "average": "= 73.32" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314820-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314820-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Wnt4 Detection Antibody", "number":"AB314820-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Wnt4 Protein Standard", "number":"AB314820-CMP11", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB314820-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314820-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Wnt4 Antibody-coated ELISA Plate", "number":"AB314820-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314820-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314820-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB314820-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ELISA Microplate Bag", "number":"AB314820-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt4 also known as Wingless-type MMTV integration site family member 4 is a protein that has significant role in cell signaling. With a molecular mass of approximately 39 kDa this protein is expressed in various tissues notably in mammalian systems such as human thyroid tissue. Wnt4 primarily facilitates communication between cells by activating signaling pathways that regulate development and differentiation processes. It is widely studied in different species including model organisms like the marmoset.
Biological function summary

Wnt4 is integral to embryonic development influencing the formation of organs and tissues. As part of the Wnt signaling pathway it works as a ligand interacting with Frizzled receptors on the cell surface. Wnt4 often in collaboration with other Wnt proteins contributes to cellular processes like proliferation migration and gene expression modulation. Its function during the development of reproductive systems and kidney morphogenesis highlights its importance in developmental biology.

Pathways

Wnt4 operates within the canonical and non-canonical Wnt signaling pathways. It primarily influences the beta-catenin signaling pathway essential for maintaining cellular homeostasis and regulating developmental signals. It can interact with other Wnt pathway components such as beta-catenin and GSK3 to modulate transcriptional activities important for cellular differentiation. By doing so it plays a pivotal role in coordinating complex signaling networks that oversee cell fate decisions.

Wnt4 displays notable associations with conditions such as Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome and polycystic ovary syndrome (PCOS). Both disorders relate to abnormalities in the reproductive system highlighting Wnt4's role in sexual development. Altered Wnt4 expression can affect other signaling proteins like beta-catenin contributing to disease pathology. By understanding its contribution to these disorders researchers aim to uncover potential therapeutic targets that rectify imbalances in Wnt signaling.
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Product protocols

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung (PubMed : 15317892, PubMed : 16959810, PubMed : 18179883, PubMed : 18182450). Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development (By similarity). Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries (PubMed : 15317892, PubMed : 16959810, PubMed : 18182450). Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary (PubMed : 15317892, PubMed : 16959810, PubMed : 18182450). Required for normal lung development and for normal patterning of trachael cartilage rings (By similarity).
See full target information WNT4
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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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