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AB314821

Human Wnt9a ELISA Kit

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Human Wnt9a ELISA Kit is a Sandwich ELISA for the measurement of Human Wnt9a in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

WNT14, WNT9A, Protein Wnt-9a, Protein Wnt-14

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Sandwich ELISA - Human Wnt9a ELISA Kit (AB314821)
  • sELISA

Supplier Data

Sandwich ELISA - Human Wnt9a ELISA Kit (AB314821)

Example of standard curve using ab314821. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.75 ng/mL

Range

0.75 - 150 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB176973

Anti-Wnt9a antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt9a antibody (AB176973)

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Proteins & Peptides

AB159814

Recombinant Human Wnt9a protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Wnt9a protein (GST tag N-Terminus) (AB159814)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Wnt9a ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Wnt9a in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Wnt9a coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "69 - 100 %", "average": "= 82.36" }, { "sample": "Plasma", "range": "67 - 83 %", "average": "= 71.73" }, { "sample": "Cell culture media", "range": "70 - 96 %", "average": "= 79.09" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314821-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB314821-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314821-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Wnt9a Detection Antibody", "number":"AB314821-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314821-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB314821-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314821-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Wnt9a Protein Standard", "number":"AB314821-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Wnt9a Antibody-coated ELISA Plate", "number":"AB314821-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314821-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt9a also known as Wingless-related integration site 9A is a secreted glycoprotein with a molecular mass of approximately 39 kDa. This protein forms part of the Wnt family which comprises signaling molecules involved in various developmental processes. Wnt9a is expressed in several tissues including the brain kidneys and skeletal tissues during embryonic development but it can also be found in adult tissues. Its expression pattern indicates a role in spatial and temporal regulation during cell growth and differentiation.
Biological function summary

Wnt9a functions in the regulation of cell proliferation migration and fate determination. It operates as part of larger protein complexes that include other Wnt family members and co-receptors such as Frizzled receptors. Wnt9a plays a significant role in osteoblast differentiation and skeletal development. It also contributes to the patterning of mesenchymal cells into chondrocytes during skeletal morphogenesis. These biological activities highlight its essential function in both developmental stages and tissue maintenance.

Pathways

Wnt9a acts through the canonical Wnt/β-catenin signaling pathway and is also involved in the non-canonical Wnt signaling pathway. Within these pathways it interacts with LRP5/6 receptors and related proteins like Dishevelled (Dvl) and Axin. The canonical pathway promotes gene transcription activation whereas the non-canonical pathway influences cell movements and organization. These interactions highlight Wnt9a’s role in the broader signaling cascades important for embryogenesis and homeostasis.

Wnt9a is associated with skeletal malformations such as Robinow syndrome and bone density disorders. Wnt9a mutations can disrupt normal skeletal development leading to abnormal growth and bone formation. In this context interactions with other proteins like BMP4 and Sox9 become disrupted contributing to the pathogenesis of these conditions. Studies continue to explore the therapeutic potential of targeting Wnt9a pathways for treating these skeletal disorders.
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Product protocols

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal timing of IHH expression during embryonic bone development, normal chondrocyte maturation and for normal bone mineralization during embryonic bone development. Plays a redundant role in maintaining joint integrity.
See full target information WNT9A
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websiteProtocolBooklet
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Product promise

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