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AB289904

Human XBP1 (Unspliced) ELISA Kit

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Human XBP1 (Unspliced) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human XBP1 (Unspliced) with a sensitivity of 3.037 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - Human XBP1 (Unspliced) ELISA Kit (AB289904)
  • sELISA

Supplier Data

Sandwich ELISA - Human XBP1 (Unspliced) ELISA Kit (AB289904)

Representative image of Human XBP1 (Unspliced) ELISA Kit (ab289904)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human XBP1 (Unspliced) Capture Antibody

- 10X Human XBP1 (Unspliced) Detector Antibody

- Human XBP1 (Unspliced) Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Sample diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human XBP1 (Unspliced) ELISA Kit (AB289904)
  • sELISA

Unknown

Sandwich ELISA - Human XBP1 (Unspliced) ELISA Kit (AB289904)

Recombinant XBP1 (Unspliced) was spiked into the following human samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. Undiluted samples are 1,000 µg/mL T-47D Cell Extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human XBP1 (Unspliced) ELISA Kit (AB289904)
  • sELISA

Unknown

Sandwich ELISA - Human XBP1 (Unspliced) ELISA Kit (AB289904)

Example of human XBP1 (Unspliced) standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

>= 3.037 pg/mL

Range

9.375 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human XBP1 (Unspliced) ELISA Kit (ab289904) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human XBP1 (Unspliced) in cell culture extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human XBP1 (Unspliced) with 3.037 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.2" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "62 - 115 %", "average": "= 86" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289904-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289904-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB289904-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289904-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289904-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289904-CMP12", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB289904-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB289904-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human XBP1 (Unspliced) Detector Antibody", "number":"AB289904-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB289904-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289904-CMP14", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human XBP1 (Unspliced) Lyophilized Recombinant Protein", "number":"AB289904-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human XBP1 (Unspliced) Capture Antibody", "number":"AB289904-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XBP1 also known as X-box binding protein 1 is a transcription factor with a mass of approximately 33 kDa. It is mainly expressed in the endoplasmic reticulum of cells where it plays a significant role in regulating the unfolded protein response (UPR). XBP1 exists in two forms: an unspliced form (XBP1u) and a spliced form (XBP1s). The splicing of XBP1 mRNA is mediated by the endoribonuclease IRE1 and is a response to endoplasmic reticulum stress. This process results in a shift of the reading frame which leads to the production of a new functional transcription factor.
Biological function summary

The action of XBP1 participates in processes critical for cell survival under stress conditions particularly in the UPR. It activates genes involved in protein folding secretion and degradation to alleviate stress within the endoplasmic reticulum. XBP1 acts as part of the larger mechanism that ensures cellular homeostasis by maintaining protein quality control. It forms a complex network with other transcription factors ensuring an integrated response to stress stimuli in various tissues including pancreatic beta cells and immune cells.

Pathways

XBP1 functions intricately within the UPR and is linked to the insulin signaling pathway. It works alongside proteins such as ATF6 and PERK to coordinate the cellular response to endoplasmic reticulum stress and sustain essential cellular processes. The IRE1-XBP1 pathway represents one arm of the UPR and shows interaction with C/EBP homologous protein (CHOP) as part of the stress signaling pathways modulating apoptosis if adaptation fails. XBP1 also influences lipid metabolism and cellular differentiation through its pathway connections impacting wide-ranging physiological processes.

Dysfunctions of XBP1 have associations with metabolic diseases and multiple myeloma. Altered XBP1 activity links to diabetes due to its role in protein homeostasis in pancreatic beta cells involving modulation of insulin secretion and sensitivity. In cancer particularly multiple myeloma XBP1 interacts with proteins such as B-cell maturation antigen (BCMA) influencing cell survival and proliferation. Targeting the XBP1 signaling axis shows potential as a therapeutic strategy for managing disorders resulting from dysregulated protein folding and stress responses.
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Product protocols

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Target data

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websiteProtocolBooklet
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Complementary Products

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