Human XCL1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human XCL1 with a sensitivity of 28.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Frontiers in medicine 10:11891342023A novel predictive model for the recurrence of pediatric alopecia areata by bioinformatics analysis and a single-center prospective study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanquan Zheng,Yingli Nie,Jingjing Lu,Hong Yi,Guili FuPubMed 37359017
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 97.48 - 2800 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 28.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Chemotactic activity for lymphocytes but not for monocytes or neutrophils. In thymus, mediates medullary accumulation of thymic dendritic cells and contributes to regulatoy T cell development, playing a role in self-tolerance establishment.
Alternative names
LTN, SCYC1, XCL1, Lymphotactin, ATAC, C motif chemokine 1, Cytokine SCM-1, Lymphotaxin, SCM-1-alpha, Small-inducible cytokine C1, XC chemokine ligand 1
What's included?
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Human XCL1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human XCL1 with a sensitivity of 28.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 97.48 - 2800 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 28.3 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 8 | mean - | SD - | C.V. 5.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 3.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 105 | Range 101 - 109 % |
Sample type Serum | Average % = 98 | Range 96 - 100 % |
Sample type Cell culture media | Average % = 90 | Range 89 - 92 % |
Sample type Heparin Plasma | Average % = 87 | Range 85 - 88 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human XCL1 ELISA Kit (ab264620) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of XCL1 protein in cell culture supernatant, hep plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human XCL1 with 28.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
XCL1 (Lymphotactin) is a secreted glycosylated protein of C chemokine family. It plays a role in chemotactic activity of lymphocytes, but not monocytes or neutrophils. In the thymus, XCL1 mediates medullary accumulation of dendritic cells and contributes to T cell development which plays a role in establishment of self-tolerance. XCL2 is functionally related XCL1 homolog, these proteins share 98% amino acids sequence identity.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
XCL1 also known as lymphotactin is a chemokine with a molecular mass of approximately 10 kDa. This protein plays a role in the immune system directing the movement of cells such as T lymphocytes to sites of inflammation or infection. XCL1 is mainly expressed in activated T cells natural killer cells and some other immune cells. Its expression gets upregulated in response to certain stimuli which helps to recruit additional immune effector cells to areas where they are needed.
- Biological function summary
XCL1 participates in chemotaxis guiding the migration of specific cells to enhance the immune response. Although XCL1 does not form part of a larger protein complex it interacts with its specific receptor XCR1 on target cells. This interaction triggers intracellular signaling pathways contributing to the controlled movement and positioning of immune cells in response to infection or inflammatory signals.
- Pathways
XCL1 is significantly involved in the chemokine signaling pathway and plays a part in immune response regulation. This pathway shared with other chemokines like CCL2 and CCL5 orchestrates the precise movement and activity of immune cells. XCL1’s interaction with XCR1 is central to initiating downstream signaling processes which influence immune responses and inflammation.
- Associated diseases and disorders
XCL1 associates with autoimmune diseases and certain viral infections. Its dysregulation appears in conditions such as rheumatoid arthritis where the abnormal control of immune cells contributes to inflammation and tissue damage. In viral infections like HIV XCL1 helps modulate immune cell responses against the virus. XCR1 the receptor of XCL1 also plays a role in these diseases being a critical component in mediating the effects of lymphotactin in varied pathological contexts.
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3 product images
Sandwich ELISA - Human XCL1 ELISA Kit (ab264620)
Human XCL1 standard curve comparison.
Standard Curve comparison between human XCL1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human XCL1 ELISA Kit (ab264620)
Example of human XCL1 standard curve in Sample Diluent NS.
The XCL1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human XCL1 ELISA Kit (ab264620)
Interpolated concentrations of native XCL1 in human PMA + Ionomycin treated PBMC cell culture supernatant samples.
Peripheral blood mononuclear cells (PBMC) were cultured in the presence (stimulated) or absence of 50 ng/mL PMA and 1 μg/mL Ionomycin for 24 hours. Cell culture supernatants were collected and assayed for the presence of XCL1. The concentrations of XCL1 were measured in duplicates, interpolated from the XCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean XCL1 concentration was determined to be 4,506 pg/mL in PMA + Ionomycin treated PBMC cell culture supernatant and not detectable in untreated samples (data not shown).
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