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AB155446

Human XIAP ELISA Kit (API3)

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Human XIAP ELISA Kit (API3) is a Sandwich ELISA for the measurement of Human XIAP (API3) in Human, Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

API3, BIRC4, IAP3, XIAP, E3 ubiquitin-protein ligase XIAP, Baculoviral IAP repeat-containing protein 4, IAP-like protein, Inhibitor of apoptosis protein 3, RING-type E3 ubiquitin transferase XIAP, X-linked inhibitor of apoptosis protein, ILP, hILP, IAP-3, hIAP-3, hIAP3, X-linked IAP

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Sandwich ELISA - Human XIAP ELISA Kit (API3) (AB155446)
  • sELISA

Unknown

Sandwich ELISA - Human XIAP ELISA Kit (API3) (AB155446)

XIAP (API3) Human ELISA Kit.

Representative standard curve using ab155446

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse, Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 0.25 ng/mL

Range

2.5 - 600 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's XIAP Human ELISA kit (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human XIAP in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human XIAP coated on a 96-well plate. Standards and samples are pipetted into the wells and XIAP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human XIAP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of XIAP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "87 - 103 %", "average": "= 95.15" }, { "sample": "Serum", "range": "74 - 90 %", "average": "= 82" }, { "sample": "Plasma", "range": "67 - 82 %", "average": "= 74.45" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155446-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155446-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB155446-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155446-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155446-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human XIAP", "number":"AB155446-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"XIAP Microplate (12 x 8 wells)", "number":"AB155446-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human XIAP Standard (lyophilized)", "number":"AB155446-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155446-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB155446-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XIAP also known as X-linked inhibitor of apoptosis protein is a member of the inhibitor of apoptosis protein (IAP) family. XIAP has a molecular mass of approximately 57 kDa. This protein inhibits apoptosis by binding to and inhibiting caspases particularly caspase-3 caspase-7 and caspase-9. XIAP expresses in a variety of tissues including the heart kidney and liver but also shows expression in cancer cells. Its role in inhibiting cell death makes it a significant focus for cancer research.
Biological function summary

The function of XIAP involves regulation of apoptosis an important process in maintaining cellular homeostasis. It forms part of the apoptosome complex by interacting with caspases to prevent premature cell death. XIAP serves to protect cells from stress-induced apoptotic signals helping in survival under adverse conditions. This ability makes it a subject of interest in studying cancer survival mechanisms since overactive XIAP can allow cancer cells to avoid programmed cell death.

Pathways

Apoptosis regulation and cell survival pathways prominently feature XIAP. Particularly XIAP plays a role in the intrinsic apoptotic pathway which is activated in response to internal stress signals. XIAP regulates this pathway via its interaction with caspase-9 and interactions with other proteins such as Smac/DIABLO which can antagonize XIAP's function. This interaction is key in balancing cell death and survival therefore highlighting its role in apoptosis control.

XIAP's dysregulation links to cancer and neurodegenerative disorders. Overexpression of XIAP can contribute to cancer by enabling cells to evade apoptosis leading to uncontrolled cell proliferation. Moreover studies have suggested a role in neurodegenerative diseases where alterations in XIAP may affect neuronal cell survival. In these contexts XIAP's interactions with proteins such as caspases and Smac/DIABLO surface as important in understanding how apoptosis evasion or enhancement contributes to disease pathology.
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Product protocols

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Target data

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis (PubMed : 11257230, PubMed : 11257231, PubMed : 11447297, PubMed : 12121969, PubMed : 12620238, PubMed : 17560374, PubMed : 17967870, PubMed : 19473982, PubMed : 20154138, PubMed : 22103349, PubMed : 9230442). Acts as a direct caspase inhibitor (PubMed : 11257230, PubMed : 11257231, PubMed : 12620238). Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry (PubMed : 11257230, PubMed : 11257231, PubMed : 16352606, PubMed : 16916640). Inactivates CASP9 by keeping it in a monomeric, inactive state (PubMed : 12620238). Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include : RIPK1, RIPK2, MAP3K2/MEKK2, DIABLO/SMAC, AIFM1, CCS, PTEN and BIRC5/survivin (PubMed : 17560374, PubMed : 17967870, PubMed : 19473982, PubMed : 20154138, PubMed : 22103349, PubMed : 22607974, PubMed : 29452636, PubMed : 30026309). Acts as an important regulator of innate immunity by mediating 'Lys-63'-linked polyubiquitination of RIPK2 downstream of NOD1 and NOD2, thereby transforming RIPK2 into a scaffolding protein for downstream effectors, ultimately leading to activation of the NF-kappa-B and MAP kinases signaling (PubMed : 19667203, PubMed : 22607974, PubMed : 29452636, PubMed : 30026309). 'Lys-63'-linked polyubiquitination of RIPK2 also promotes recruitment of the LUBAC complex to RIPK2 (PubMed : 22607974, PubMed : 29452636). Regulates the BMP signaling pathway and the SMAD and MAP3K7/TAK1 dependent pathways leading to NF-kappa-B and JNK activation (PubMed : 17560374). Ubiquitination of CCS leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation (PubMed : 20154138). Ubiquitination of MAP3K2/MEKK2 and AIFM1 does not lead to proteasomal degradation (PubMed : 17967870, PubMed : 22103349). Plays a role in copper homeostasis by ubiquitinating COMMD1 and promoting its proteasomal degradation (PubMed : 14685266). Can also function as E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation (PubMed : 21145488). Ubiquitinates and therefore mediates the proteasomal degradation of BCL2 in response to apoptosis (PubMed : 29020630). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner (PubMed : 22095281). Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8 (PubMed : 22095281). Acts as a positive regulator of Wnt signaling and ubiquitinates TLE1, TLE2, TLE3, TLE4 and AES (PubMed : 22304967). Ubiquitination of TLE3 results in inhibition of its interaction with TCF7L2/TCF4 thereby allowing efficient recruitment and binding of the transcriptional coactivator beta-catenin to TCF7L2/TCF4 that is required to initiate a Wnt-specific transcriptional program (PubMed : 22304967).
See full target information XIAP
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