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AB313509

Human XPNPEP2/mAPP ELISA Kit

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Human XPNPEP2/mAPP ELISA Kit is a Sandwich ELISA for the measurement of Human XPNPEP2/mAPP in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Xaa-Pro aminopeptidase 2, Aminoacylproline aminopeptidase, Membrane-bound aminopeptidase P, X-Pro aminopeptidase 2, Membrane-bound APP, Membrane-bound AmP, mAmP, XPNPEP2

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Sandwich ELISA - Human XPNPEP2/mAPP ELISA Kit (AB313509)
  • sELISA

Supplier Data

Sandwich ELISA - Human XPNPEP2/mAPP ELISA Kit (AB313509)

Example of standard curve using ab313509. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.62 ng/mL

Range

0.62 - 150 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB224034

Anti-XPNPEP2/mAPP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP2/mAPP antibody (AB224034)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human XPNPEP2/mAPP ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human XPNPEP2/mAPP in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human XPNPEP2/mAPP coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human 4-1BB Ligand (TNFSF9), Activin RIIB, BAMBI (NMA), BOC, Brevican, Carbonic Anhydrase XII, Carboxypeptidase A2, CD300c (LMIR2), CD320 (TCblR, 8D6A), CDNF, CDO, CHST1, CHST4, CILP1, CNTF R alpha, CRIM1, CRTAC1, CXADR, Dopa Decarboxylase (DDC), DPPII (QPP, DPP7), DSPG3, EMR2, FCAR (CD89), FCRL1 (FcRH1), FCRL2 (FcRH2), Gas6, GPR56, GPVI, Hepsin, ILT2 (CD85j), Jagged 2, Kirrel3 (NEPH2), KLF4, LAIR1, LAMP, LAMP1 (CD107a), MDGA1, MIS RII, Neurexin 3.

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Recovery

[ { "sample": "Cell culture media", "range": "75 - 89 %", "average": "= 82.12" }, { "sample": "Plasma", "range": "77 - 92 %", "average": "= 83.98" }, { "sample": "Serum", "range": "73 - 86 %", "average": "= 79.44" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XPNPEP2 also known as membrane-bound aminopeptidase P or mAPP is an enzyme with a mass of approximately 74 kDa. This protein acts by cleaving the N-terminal amino acid from peptides where proline is the penultimate residue. It is an integral membrane protein mainly expressed in endothelial cells and is also detectable in the kidneys heart and liver. The primary function of XPNPEP2 involves peptide processing reflecting a specific enzymatic mechanism.
Biological function summary

This enzyme plays an important role in the degradation of bioactive peptides such as bradykinin. XPNPEP2 does not function as part of a larger protein complex but exhibits specific affinity toward substrates with proline residues. The enzymatic activity ensures the regulation of peptide hormones and other vasoactive compounds within the vascular system. Proper functioning of this target ensures balanced peptide levels affecting physiological processes primarily related to blood pressure regulation.

Pathways

The involvement of XPNPEP2 in the kallikrein-kinin system gives it a role in cardiovascular physiology. It interacts with proteins like kininogen and kallikrein which are critical components of this pathway. XPNPEP2 aids in modulating the activity of bradykinin a peptide known for its vasodilatory properties. Additionally the enzyme links to nitrogen metabolism sharing pathways with aminopeptidase families which are involved in metabolizing nitrogen-rich compounds.

Deficiencies or dysfunctions related to XPNPEP2 can contribute to conditions such as hereditary angioedema and hypertension. The relationship of XPNPEP2 with proteins like C1 inhibitor has implications in the former disease where improper regulation of vasoactive peptides leads to uncontrolled vascular permeability and swelling. In hypertension alterations in bradykinin metabolism caused by dysregulated XPNPEP2 activity may exacerbate or contribute to elevated blood pressure states. Understanding these interactions helps in identifying therapeutic targets for treatment and management.
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Product protocols

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Target data

Membrane-bound metalloprotease which catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. May play a role in the metabolism of the vasodilator bradykinin.
See full target information XPNPEP2
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websiteProtocolBooklet
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Product promise

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