AB269544

Human YB1 ELISA Kit (YBX1)

Recombinant
SimpleStep
What is this?

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(1 Publication)

Human YB1 ELISA Kit (YBX1) is a single-wash 90-min Simplestep used to quantify Human YB1 (YBX1) with a sensitivity of 0.85 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

NSEP1, YB1, YBX1, Y-box-binding protein 1, YB-1, CCAAT-binding transcription factor I subunit A, DNA-binding protein B, Enhancer factor I subunit A, Nuclease-sensitive element-binding protein 1, Y-box transcription factor, CBF-A, DBPB, EFI-A

2 Images

Sandwich ELISA - Human YB1 ELISA Kit (YBX1) (AB269544)

sELISA

Supplier Data

Sandwich ELISA - Human YB1 ELISA Kit (YBX1) (AB269544)

Interpolated concentrations of native YB1 in human Hela cell extract.

YB1 in human Hela cell extract based on a 25 μg/mL extract load. The concentrations of YB1 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human YB1 ELISA Kit (YBX1) (AB269544)

Example of human YB1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

The YB1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.85 ng/mL

Range

0.9 - 60 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human YB1 ELISA Kit (YBX1) (ab269544) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of YB1 (YBX1) protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human YB1 (YBX1) with 0.85 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Y box binding protein 1 (YB1) or nuclease-sensitive element-binding protein 1 is a DNA- and RNA- binding protein encoded by the *YBX1* gene. YB1 binds preferentially to the 5'-[CU]CUGCG-3' RNA motif and specifically recognizes mRNA transcripts modified by C5-methylcytosine (m5C). YB1 is involved in translational repression, RNA stabilization, mRNA splicing, DNA repair, and transcription regulation.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "106 - 120 %", "average": "= 114" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab269544-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB269544-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB269544-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB269544-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB269544-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human YB1 (YBX1) Capture Antibody", "number":"AB269544-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human YB1 (YBX1) Lyophilized Recombinant Protein", "number":"AB269544-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB269544-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB269544-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB269544-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human YB1 (YBX1) Detector Antibody", "number":"AB269544-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB269544-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Y-box Binding Protein 1 (YB1) also known as YBX1 is a multifunctional protein with a mass of approximately 36 kDa. It belongs to the cold shock protein family that contains a highly conserved cold shock domain. YB1 is broadly expressed across many tissues predominantly found in the cytoplasm and nucleus where it functions as a transcription factor and RNA-binding protein. Through its transcriptional regulatory activity YB1 can bind to Y-box sequences in the promoter regions of genes and modulate their expression.

Biological function summary

YB1 plays vital roles in regulating cell proliferation stress response and differentiation. It does not operate alone; it interacts with various complexes notably ribonucleoprotein complexes. YB1 regulates gene expression at both transcriptional and translational levels impacting cell cycle progression and apoptosis pathways. Its ability to bind RNA and DNA makes it integral in controlling the expression of genes related to stress responses and developmental processes.

Pathways

YB1 is essential in many signaling pathways that govern cell growth and survival including the PI3K/AKT pathway and MAPK pathway. In these pathways it interacts with proteins like AKT1 and MAPK3 influencing cellular responses to external and internal stimuli. These interactions reveal the adaptability of YB1 in various cellular contexts allowing it to mediate pathway-specific responses to environmental cues thereby promoting the maintenance of cellular homeostasis and adaptation.

The dysregulation of YB1 has been linked to cancer and neurodegenerative diseases. Overexpression or altered localization of YB1 often in connection with other proteins such as p53 contributes to tumorigenesis by enhancing cell proliferation and inhibiting programmed cell death. In neurodegenerative diseases impaired YB1 function can negatively affect neuronal survival potentially mediated through its interactions with proteins like tau which are implicated in conditions such as Alzheimer's disease. Understanding of these associations highlights the potential of YB1 as a therapeutic target in disease management.

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Product protocols

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Target data

DNA- and RNA-binding protein involved in various processes, such as translational repression, RNA stabilization, mRNA splicing, DNA repair and transcription regulation (PubMed : 10817758, PubMed : 11698476, PubMed : 14718551, PubMed : 18809583, PubMed : 31358969, PubMed : 8188694). Predominantly acts as a RNA-binding protein : binds preferentially to the 5'-[CU]CUGCG-3' RNA motif and specifically recognizes mRNA transcripts modified by C5-methylcytosine (m5C) (PubMed : 19561594, PubMed : 31358969). Promotes mRNA stabilization : acts by binding to m5C-containing mRNAs and recruiting the mRNA stability maintainer ELAVL1, thereby preventing mRNA decay (PubMed : 10817758, PubMed : 11698476, PubMed : 31358969). Component of the CRD-mediated complex that promotes MYC mRNA stability (PubMed : 19029303). Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Plays a key role in RNA composition of extracellular exosomes by defining the sorting of small non-coding RNAs, such as tRNAs, Y RNAs, Vault RNAs and miRNAs (PubMed : 27559612, PubMed : 29073095). Probably sorts RNAs in exosomes by recognizing and binding C5-methylcytosine (m5C)-containing RNAs (PubMed : 28341602, PubMed : 29073095). Acts as a key effector of epidermal progenitors by preventing epidermal progenitor senescence : acts by regulating the translation of a senescence-associated subset of cytokine mRNAs, possibly by binding to m5C-containing mRNAs (PubMed : 29712925). Also involved in pre-mRNA alternative splicing regulation : binds to splice sites in pre-mRNA and regulates splice site selection (PubMed : 12604611). Binds to TSC22D1 transcripts, thereby inhibiting their translation and negatively regulating TGF-beta-mediated transcription of COL1A2 (By similarity). Also able to bind DNA : regulates transcription of the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7' (PubMed : 18809583). Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes (PubMed : 18809583, PubMed : 8188694). Promotes separation of DNA strands that contain mismatches or are modified by cisplatin (PubMed : 14718551). Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA, suggesting a role in DNA repair (PubMed : 14718551). The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation (PubMed : 19483673).

See full target information YBX1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 13:e12417 PubMed38499475

2024

Y-box binding protein 1 in small extracellular vesicles reduces mesenchymal stem cell differentiation to osteoblasts-implications for acute myeloid leukaemia.

Applications

Unspecified application

Species

Unspecified reactive species

Venkatesh Kumar Chetty,Jamal Ghanam,Kristína Lichá,Alexandra Brenzel,Dirk Reinhardt,Basant Kumar Thakur

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human YB1 ELISA Kit (YBX1)

Sandwich ELISA - Human YB1 ELISA Kit (YBX1) (AB269544)

Sandwich ELISA - Human YB1 ELISA Kit (YBX1) (AB269544)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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