Human YKL-40 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human YKL-40 in Cerebral Spinal Fluid, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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- Aging 15:1199-12092023Prognostic performance of serum YKL-40 for one-year clinical outcomes in acute ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuomei Shi,Minghao Li,Yan E,Meng Wang,Pengyu Gong,Xiaorong Wang,Jingye Lu,Weixiang Wu,Shouru Xue,Junshan Zhou,Rujuan ZhouPubMed 36880855
- Journal of inflammation research 16:311-3192023Serum YKL-40 Levels and White Matter Hyperintensities in Patients with Acute Ischemic Stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuomei Shi,Dongdong Ke,Pengyu Gong,Peng Yu,Junshan Zhou,Meng Wang,Xiaohao Zhang,Xiaorong Wang,Minwang Guo,Mingyang Xu,Rujuan ZhouPubMed 36721527
- Brain, behavior, & immunity - health 26:1005552022Conserved YKL-40 changes in mice and humans after postoperative delirium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer David-Bercholz,Leah Acker,Ana I Caceres,Pau Yen Wu,Saanvi Goenka,Nathan O Franklin,Ramona M Rodriguiz,William C Wetsel,Michael Devinney,Mary Cooter Wright,Henrik Zetterberg,Ting Yang,Miles Berger,Niccolò TerrandoPubMed 36457825
- Journal of inflammation research 14:4361-43692021The Relationship Between Serum YKL-40 Levels on Admission and Stroke-Associated Pneumonia in Patients with Acute Ischemic Stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuomei Shi,Wenxiu Chen,Pengyu Gong,Meng Wang,Junshan Zhou,Xiaorong Wang,Minwang Guo,Jingye Lu,Yan Li,Hongxuan Feng,Xuetao Fu,Rujuan Zhou,Shouru XuePubMed 34511972
Key facts
- Detection method
Colorimetric
- Sample types
Cerebral Spinal Fluid, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human, Rhesus monkey
- Range
23.44 - 1500 pg/mL
- Assay time
1h 30m
- Sensitivity
= 3.9 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
Alternative names
Chitinase-3-like protein 1, 39 kDa synovial protein, Cartilage glycoprotein 39, YKL-40, CGP-39, GP-39, hCGP-39, CHI3L1
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Human YKL-40 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human YKL-40 in Cerebral Spinal Fluid, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Cerebral Spinal Fluid, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human, Rhesus monkey
- Range
23.44 - 1500 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 3.9 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 1.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 103 | Range 100 - 107 % |
Sample type Serum | Average % = 110 | Range 101 - 122 % |
Sample type EDTA Plasma | Average % = 118 | Range 113 - 124 % |
Sample type Cerebral Spinal Fluid | Average % = 105 | Range 101 - 110 % |
Sample type Urine | Average % = 102 | Range 98 - 109 % |
Sample type Heparin Plasma | Average % = 108 | Range 103 - 118 % |
Sample type Citrate plasma | Average % = 116 | Range 113 - 123 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human YKL-40 ELISA Kit (ab255719) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of YKL-40 protein in serum, plasma, urine, cell culture supernatant and cerebrospinal fluid. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human YKL-40 with 3.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
YKL-40 (Chitinase-3-like protein 1) is a carbohydrate binding lectin with a preference for chitin. YKL-40 may play a role in tissue remodeling and cell response to changes in environment. It plays a the Th2 response of T-helper cells and IL-13-induced inflammation by regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation, and M2 macrophage differentiation. It regulated antibacterial response in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
YKL-40 also known as chitinase-3-like protein 1 (CHI3L1) is a glycoprotein with a molecular weight of approximately 40 kDa. Scientists often study it for its roles in inflammatory processes. It has expressions in numerous tissues including liver cartilage and the central nervous system. The CHI3L1 protein does not possess chitinase activity unlike other members of the chitinase family yet it interacts with various ligands to influence cellular behavior.
- Biological function summary
YKL-40 plays significant roles in tissue remodeling and inflammation. It does not form part of a larger complex by itself but influences several cellular processes. This protein affects cell proliferation migration and survival making it involved in both normal physiological processes and pathological conditions. CHI3L1 also exhibits a connection to the immune response where it modulates macrophage activity and interacts with other immune cells.
- Pathways
YKL-40 is integral to the inflammatory and extracellular matrix pathways. It associates with proteins such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) influencing signaling cascades that modulate inflammation. Through these interactions CHI3L1 becomes a mediator of connective tissue metabolism and immune reactions in various pathophysiological states.
- Associated diseases and disorders
YKL-40 shows strong links to conditions like osteoarthritis and certain cancers. In osteoarthritis elevated levels of CHI3L1 reflect increased cartilage degradation while in oncology it correlates with tumor angiogenesis and metastasis potential. Its connection with proteins such as matrix metalloproteinases highlights its role in disease mechanisms highlighting its potential as a biomarker for early detection and disease progression monitoring.
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5 product images
Sandwich ELISA - Human YKL-40 ELISA Kit (ab255719)
Human YKL-40 standard curve comparison.
Standard Curve comparison between human YKL-40 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human YKL-40 ELISA Kit (ab255719)
Example of human YKL-40 standard curve in Sample Diluent NS.
The YKL-40 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human YKL-40 ELISA Kit (ab255719)
Interpolated concentrations of native YKL-40 in human serum and plasma samples.
The concentrations of YKL-40 were measured in duplicates, interpolated from the YKL-40 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:40, plasma (citrate) 1:20, plasma (heparin) 1:20, and plasma (EDTA) 1:80. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 37.59 ng/mL in neat serum, 16.17 ng/mL in neat plasma (citrate), 18.92 in neat plasma (heparin), and 67.92 ng/mL in neat plasma (EDTA).
Sandwich ELISA - Human YKL-40 ELISA Kit (ab255719)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 42.74 ng/mL with a range of 16.87 – 78.32 ng/mL.
Sandwich ELISA - Human YKL-40 ELISA Kit (ab255719)
Interpolated concentrations of native YKL-40 in human urine, cell culture supernatant and cerebrospinal fluid (CSF) samples.
The concentrations of YKL-40 were measured in duplicates, interpolated from the YKL-40 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 100%, HepG2 cell culture supernatant 100%, U-87 MG cell culture supernatant 5%, and CSF 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 449.02 pg/mL in neat urine, 123.48 pg/mL in neat HepG2 cell culture supernatant, 18,524 pg/mL in neat U-87 MG cell culture supernatant and 145,710 pg/mL in neat CSF.
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