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AB314822

Human YWHAE ELISA Kit

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Human YWHAE ELISA Kit is a Sandwich ELISA for the measurement of Human YWHAE in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

14-3-3 protein epsilon, 14-3-3E, YWHAE

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Sandwich ELISA - Human YWHAE ELISA Kit (AB314822)
  • sELISA

Supplier Data

Sandwich ELISA - Human YWHAE ELISA Kit (AB314822)

Example of standard curve using ab314822. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.34 ng/mL

Range

0.34 - 80 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB92311

Anti-YWHAE antibody [EPR3918]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-YWHAE antibody [EPR3918] (AB92311)

  • 5
  • 2
Proteins & Peptides

AB54317

Recombinant Human YWHAE protein (Tag Free)

SDS-PAGE - Recombinant Human YWHAE protein (Tag Free) (AB54317)

  • 0
  • 0
Cell Lines & Lysates

AB274939

Human YWHAE knockout HeLa cell line

Next Generation Sequencing - Human YWHAE knockout HeLa cell line (AB274939)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human YWHAE ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human YWHAE in serum (human YWHAE concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human YWHAE coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "73 - 120 %", "average": "= 88.91" }, { "sample": "Plasma", "range": "68 - 79 %", "average": "= 73.13" }, { "sample": "Cell culture media", "range": "70 - 114 %", "average": "= 84.57" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314822-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314822-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314822-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314822-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314822-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human YWHAE Detection Antibody", "number":"AB314822-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human YWHAE Protein Standard", "number":"AB314822-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human YWHAE Antibody-coated ELISA Plate", "number":"AB314822-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"350X HRP-Streptavidin", "number":"AB314822-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB314822-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Plate Sealing Film", "number":"AB314822-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The YWHAE protein also known as 14-3-3E acts as a regulatory molecule by binding to various phosphoserine-containing proteins. It plays key roles in cellular signaling and exists as a dimer. YWHAE has a molecular mass of around 29 kDa and is widely expressed in tissues including the brain heart and skeletal muscle. Its interactions often rely on its abundant presence in various cell types showing its versatility.
Biological function summary

YWHAE protein interacts with several enzymes and receptors forming complexes that affect their functional states. It is a member of the 14-3-3 family which facilitates signal transduction by binding to phosphorylated serine/threonine residues. This protein influences processes like cell cycle control apoptosis and stress responses emphasizing its significant roles in maintaining cellular homeostasis.

Pathways

YWHAE plays significant roles in the Akt and MAPK pathways. In the Akt pathway YWHAE partners with proteins like BAD and FOXO which are important in controlling apoptosis and cell survival. In the MAPK pathway it regulates multiple proteins including Raf and MEK impacting cell growth and differentiation. Understanding YWHAE's interactions in these pathways highlights its influence in critical signaling events.

YWHAE has been tied to schizophrenia and neurodevelopmental disorders. In schizophrenia it interacts with proteins like DISC1 affecting neuronal migration and synaptic function. In neurodevelopmental disorders alterations in YWHAE can lead to compromised signaling that disrupts normal brain function. These connections highlight the importance of YWHAE in maintaining neurological health.
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Product protocols

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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 21189250). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 35343654). Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed : 12917326). Plays a positive role in the antiviral signaling pathway upstream of TBK1 via interaction with RIGI (PubMed : 37555661). Mechanistically, directs RIGI redistribution from the cytosol to mitochondrial associated membranes where it mediates MAVS-dependent innate immune signaling during viral infection (PubMed : 22607805). Plays a role in proliferation inhibition and cell cycle arrest by exporting HNRNPC from the nucleus to the cytoplasm to be degraded by ubiquitination (PubMed : 37599448).
See full target information YWHAE
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Product promise

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