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AB277419

Human Zinc Alpha 2 Glycoprotein ELISA kit

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Human Zinc Alpha 2 Glycoprotein ELISA kit is a Sandwich (quantitative) ELISA for the measurement of Human Zinc Alpha 2 Glycoprotein in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Zinc-alpha-2-glycoprotein, Zn-alpha-2-GP, Zn-alpha-2-glycoprotein, Azgp1

1 Images
ELISA - Human Zinc Alpha 2 Glycoprotein ELISA kit (AB277419)
  • ELISA

Supplier Data

ELISA - Human Zinc Alpha 2 Glycoprotein ELISA kit (AB277419)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.04 ng/mL

Range

0.041 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Zinc Alpha 2 Glycoprotein ELISA Kit (ab277419) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Zinc Alpha 2 Glycoprotein in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human Zinc Alpha 2 Glycoprotein coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Zinc Alpha 2 Glycoprotein present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Zinc Alpha 2 Glycoprotein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Zinc Alpha 2 Glycoprotein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "73 - 84 %", "average": "= 78.73" }, { "sample": "Plasma", "range": "76 - 85 %", "average": "= 79.34" }, { "sample": "Cell culture media", "range": "70 - 78 %", "average": "= 74.51" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277419-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277419-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277419-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Zinc Alpha 2 Glycoprotein coated Microplate", "number":"AB277419-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277419-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Zinc Alpha 2 Glycoprotein standard protein (Lyophilized)", "number":"AB277419-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"900x HRP-Streptavidin Concentrate", "number":"AB277419-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Zinc Alpha 2 Glycoprotein Antibody", "number":"AB277419-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277419-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zinc Alpha 2 Glycoprotein (ZAG) also called ZAG or AZGP1 is a soluble protein with a molecular mass of approximately 41 kDa. It functions as an adipokine and is mostly found in the liver breast and prostate tissues as well as in bodily fluids like plasma and saliva. ZAG belongs to the major histocompatibility complex class I family and it shares structural similarities with antigen-presenting proteins.
Biological function summary

Zinc Alpha 2 Glycoprotein assists in lipid metabolism. It promotes the breakdown of triglycerides in adipose tissue by stimulating the beta-adrenergic pathway. ZAG functions independently and does not typically form complexes with other proteins during this process. Its presence in adipose tissue suggests a role in energy homeostasis and body weight regulation.

Pathways

Zinc Alpha 2 Glycoprotein participates in the metabolic pathway for lipid degradation. The protein interacts with beta-3 adrenergic receptors to trigger lipolysis which involves breaking down fat cells into free fatty acids and glycerol. Its action in this pathway links ZAG with other proteins such as perilipin which protects lipid droplets from breakdown.

Zinc Alpha 2 Glycoprotein has connections with metabolic-related conditions notably obesity and cancer cachexia. In obesity lower levels of ZAG are often observed potentially linking it to impaired fat metabolism. In cancer cachexia elevated ZAG levels are frequently detected suggesting a role in the loss of fat and muscle tissue. ZAG's interaction with certain cytokines may enhance cachexia progression by affecting protein degradation pathways.
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Product protocols

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Target data

Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers.
See full target information Azgp1
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websiteProtocolBooklet
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