AB287832

Inositol Triphosphate (IP3) ELISA Kit

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Inositol Triphosphate (IP3) ELISA Kit is a Competitive ELISA for the measurement of Inositol Triphosphate (IP3) in in Biofluids samples.

View Alternative Names

Inositol-tetrakisphosphate 1-kinase, Inositol-triphosphate 5/6-kinase, ITPK1

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Functional Studies - Inositol Triphosphate (IP3) ELISA Kit (AB287832)

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Functional Studies - Inositol Triphosphate (IP3) ELISA Kit (AB287832)

Inositol Triphosphate (IP3) ELISA Kit.

Typical standard curve using ab287832. For reference use only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Assay type

Competitive

Sensitivity

= 9.38 pg/mL

Range

15.63 - 1000 pg/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

This IP3 ELISA Kit ab287832 (previoulsy known as Inositol Triphosphate (IP3) ELISA kit, E4792) is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with IP3. During the reaction, IP3 in the samples or standard competes with IP3 coated on the plate for binding to the anti-IP3 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of IP3. The concentration of IP3 in the samples can be calculated by comparing the OD of the samples to the standard curve.

This kit recognizes IP3 in samples. No Significant cross-reactivity or interference between IP3 and analogues was observed.

This product is manufactured by BioVision, an Abcam company and was previously called E4792 Inositol Triphosphate (IP3) ELISA Kit. E4792-100 is the same size as the 96 test size of ab287832.

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Recovery

[ { "sample": "Serum", "range": "88 - 100 %", "average": "= 93" }, { "sample": "Cell culture media", "range": "85 - 101 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "88 - 98 %", "average": "= 93" } ]

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab287832-96Test", "assetComponentDetails": [ { "size":"4 x 1 Unit", "name":"Plate Sealer", "number":"AB287832-CMP05" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB287832-CMP11" }, { "size":"1 x 14 mL", "name":"Biotinylated Detection Antibody Diluent", "number":"AB287832-CMP04" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB287832-CMP09" }, { "size":"1 x 10 mL", "name":"Substrate Reagent", "number":"AB287832-CMP10" }, { "size":"1 x 14 mL", "name":"HRP-Conjugate Diluent", "number":"AB287832-CMP02" }, { "size":"1 x 20 mL", "name":"Reference Sample and Standard Diluent", "number":"AB287832-CMP06" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB287832-CMP08" }, { "size":"1 x 120 µL", "name":"100X HRP-Conjugate", "number":"AB287832-CMP01" }, { "size":"2 x 1 Vial", "name":"Reference Standard", "number":"AB287832-CMP07" }, { "size":"1 x 120 µL", "name":"100X Biotinylated Detection Antibody", "number":"AB287832-CMP03" } ] }, "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab287832-96Test", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB287832-CMP11", "productcode":"" }, { "size":"1 x 14 mL", "name":"Biotinylated Detection Antibody Diluent", "number":"AB287832-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB287832-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB287832-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"Substrate Reagent", "number":"AB287832-CMP10", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X HRP-Conjugate", "number":"AB287832-CMP01", "productcode":"" }, { "size":"1 x 14 mL", "name":"HRP-Conjugate Diluent", "number":"AB287832-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"Reference Sample and Standard Diluent", "number":"AB287832-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Reference Standard", "number":"AB287832-CMP07", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X Biotinylated Detection Antibody", "number":"AB287832-CMP03", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Sealer", "number":"AB287832-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The enzyme inositol 134-trisphosphate 5/6-kinase commonly known as ITPK1 catalyzes the phosphorylation of inositol trisphosphate (IP3) to produce higher phosphorylated forms of inositol phosphates. ITPK1 has a molecular mass of approximately 47 kDa and expresses in various human tissues though it is highly expressed in the brain and skeletal muscles. Researchers often measure ITPK1 activity using inositol trisphosphate (IP3) assays including IP3 ELISA and IP3 measurement methods.

Biological function summary

ITPK1 is involved in regulating cellular levels of inositol phosphates acting as a significant node in the inositol signaling pathways. As a part of a kinase complex it modulates cellular processes like signal transduction osmoregulation and cell growth. ITPK1's role in controlling IP3 levels positions it as a contributor to maintaining cellular homeostasis and enabling proper cellular responses to external stimuli.

Pathways

ITPK1 plays an essential role in the IP3 pathway by converting inositol trisphosphate (IP3) into inositol tetrakisphosphate (IP4). This pathway influences calcium release within cells impacting various physiological processes. ITPK1 is associated with pivotal signaling proteins like IP3 receptors which bind with IP3 to trigger intracellular calcium release playing an important role in cellular signaling and communication networks.

Mutations or dysfunctions in ITPK1 have links to conditions like schizophrenia and Down syndrome. Schizophrenia may relate to altered inositol phosphate metabolism wherein interactions with proteins like IP3 receptors are disrupted. ITPK1 also shows a connection to Alzheimer's disease due to its role in calcium signaling pathways which influences neuronal function and survival. These associations highlight ITPK1's potential significance in neuropsychiatric and neurodegenerative diseases.

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Product protocols

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Target data

Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3 (PubMed : 11042108, PubMed : 8662638). Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5 (PubMed : 11042108). This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway (PubMed : 11042108, PubMed : 8662638). Also acts as an inositol polyphosphate phosphatase that dephosphorylates Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4 (PubMed : 11909533, PubMed : 17616525). May also act as an isomerase that interconverts the inositol tetrakisphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium (PubMed : 11909533). Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain (PubMed : 11909533, PubMed : 12925536, PubMed : 17616525). Plays an important role in MLKL-mediated necroptosis. Produces highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which bind to MLKL mediating the release of an N-terminal auto-inhibitory region leading to its activation. Essential for activated phospho-MLKL to oligomerize and localize to the cell membrane during necroptosis (PubMed : 17616525).

See full target information ITPK1

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Product promise

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Inositol Triphosphate (IP3) ELISA Kit

Functional Studies - Inositol Triphosphate (IP3) ELISA Kit (AB287832)

Key facts

Detection method

Sample types

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise