AB176662

JNK 1/2 (pT183/Y185 + Total) ELISA Kit

Name: JNK 1/2 (pT183/Y185 + Total) ELISA Kit (ab176662) | Abcam
Brand: Abcam Limited
SKU: AB176662
Price: 725 USD
Availability: InStock
Rating: 5 (2 reviews)

SimpleStep
What is this?

(2 Reviews)

(14 Publications)

JNK 1/2 (pT183/Y185 + Total) ELISA Kit is a single-wash 90-min Simplestep used to detect JNK 1/2 (pT183/Y185 + Total). The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Semi-quantitative Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 10 citations

6 Images

ELISA - JNK 1/2 (pT183/Y185 + Total) ELISA Kit (AB176662)

ELISA

Supplier Data

ELISA - JNK 1/2 (pT183/Y185 + Total) ELISA Kit (AB176662)

sELISA

Supplier Data

Sandwich ELISA - JNK 1/2 (pT183/Y185 + Total) ELISA Kit (AB176662)

JNK1/2 (pT183/Y185) phosphorylation in response to anisomycin treatment.

Induction of JNK1/2 (pT183/Y185) phosphorylation in HeLa cells in response to anisomycin treatment. HeLa cells were cultured in 96-well tissue culture plates and treated (30 min) with a dose-range of anisomycin before cell lysis. Data from quadruplicate measurements of JNK1/2 (pT183/Y185) are plotted and compared against Total JNK1/2 protein levels. Comparative JNK1/2 (pT183/Y185) and JNK1/2 (Total) data also shown by Western Blot.

sELISA

Supplier Data

Sandwich ELISA - JNK 1/2 (pT183/Y185 + Total) ELISA Kit (AB176662)

Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1X Cell Extraction Buffer PTR. Data from duplicate measurements of JNK1/2 (pT183/Y185) are normalized and plotted.

sELISA

Supplier Data

Sandwich ELISA - JNK 1/2 (pT183/Y185 + Total) ELISA Kit (AB176662)

Typical cell lysate dilution series.

Example of a typical JNK1/2 (pT183/Y185) and JNK1/2 (Total) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - JNK 1/2 (pT183/Y185 + Total) ELISA Kit (AB176662)

Typical recombinant protein standard curve.

Example of a typical JNK1/2 (pT183/Y185) recombinant protein standard curve. The proportion of total protein that is phosphorylated is unknown - data is indicative only. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - JNK 1/2 (pT183/Y185 + Total) ELISA Kit (AB176662)

Comparison of JNK1/2 expression in different cell lines.

Cell line analysis for Total JNK1/2 from 200 μg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).

Key facts

Detection method

Colorimetric

Sample types

Tissue Homogenate, Cell Lysate

Reacts with

Mouse, Human

Assay type

Semi-quantitative

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's JNK1/2 (pT183/Y185) and JNK1/2 (Total) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of JNK1/2 (pT183/Y185) and Total JNK1/2 protein in Human and mouse cells.

The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

As of October 2019, this kit was reformulated with new antibodies to maintain continued long term supply.

Estimated sensitivity: Phospho-JNK1/2 (Thr183/Tyr185): 0.5 ng/mL (tested with recombinant protein), Total JNK1/2: 0.1 ng/mL (tested with recombinant protein)
Range: Phospho-JNK1/2 (Thr183/Tyr185): 1-100 ng/mL, Total JNK1/2: 0.2-20 ng/mL

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "(pT183/Y185)", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Intra", "sample": "(pT183/Y185)", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab176662-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1.5 mL", "name":"JNK1/2 (pT183/Y185) Capture Antibody", "number":"AB176662-CMP04", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"JNK1/2 (Total) Capture Antibody", "number":"AB176662-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Wash Buffer PT", "number":"AB176662-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized JNK1/2 Control Lysate", "number":"AB176662-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution", "number":"AB176662-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"5X Cell Extraction Buffer PTR", "number":"AB176662-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB176662-CMP10", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"JNK1/2 (pT183/Y185) Detector Antibody", "number":"AB176662-CMP05", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"JNK1/2 (Total) Detector Antibody", "number":"AB176662-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB176662-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB176662-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB176662-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JNK1 and JNK2 also known as Stress-activated Protein Kinases (SAPK) are members of the Mitogen-Activated Protein Kinase (MAPK) family. JNK1 and JNK2 are encoded by the MAPK8 and MAPK9 genes respectively. These kinases are about 46-54 kDa in size and are predominantly expressed in a wide variety of tissues including the brain liver and heart. JNK1 and JNK2 play a central role in transducing signals triggered by stress cytokines and growth factors. They become activated in response to various cellular stresses and are known for their involvement in the regulation of transcription factors such as c-Jun.

Biological function summary

The proteins function as critical regulators in cellular processes such as apoptosis inflammation and cell differentiation. JNK1 and JNK2 can form complexes with other proteins to mediate their effects impacting gene expression by phosphorylating transcription factors. These kinases have been extensively studied through techniques like JNK ELISA which measure their activity levels under various conditions. JNK1 and JNK2 are key components in cellular responses to stress and can trigger different outcomes depending on the context and stimuli.

Pathways

The JNK1 and JNK2 proteins integrate into the MAPK signaling cascade which influences diverse cellular activities. They participate in the c-Jun N-terminal kinase pathway an important route affecting the transcription of genes involved in apoptosis. This pathway interacts with proteins such as p53 and ATF2 which further link JNK activity to cellular stress responses. Additionally JNKs contribute to the inflammatory responses by collaborating with NF-kB and other related proteins.

JNK1 and JNK2 have significant roles in the progression of cancer and neurodegenerative disorders like Alzheimer's disease. Aberrant activity or expression of these kinases can lead to enhanced tumorigenesis partly through their interaction with proteins like p53 which regulate cell cycle and apoptosis. In Alzheimer's disease JNKs associate with the pathology by affecting tau phosphorylation linked to neuronal death. Understanding these connections can aid in developing therapeutic strategies targeting JNK pathways.

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Product protocols

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Target data

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 44:2677-2688 PubMed40394415

2025

A multi-faceted discovery strategy identifies functional antibodies binding to cysteine-rich domain 1 of hDKK1 for cancer immunotherapy via Wnt non-canonical pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Linya Wang,Sean M Peterson,Marisa Yang,Ana G Lujan Hernandez,Tom Z Yuan,Zhen Han,Vishwas Prabhu,Kara Y Chan,Cameron F Hu,Mouna Villalta,Tammy Htoy,Paul VanDyke,Carson Holliday,Hector Franco,Hansika Wadhwa,Hoa Giang,Ryan Stafford,Fumiko Axelrod,Aaron Sato

PloS one 18:e0286454 PubMed37352173

2023

Aged black garlic extract inhibits the growth of estrogen receptor-positive breast cancer cells by downregulating MCL-1 expression through the ROS-JNK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiwei Yang,Fang Li,Guohui Jia,Rui Liu

Heliyon 9:e15805 PubMed37251857

2023

The topoisomerase inhibitor CPT-11 prevents the growth and metastasis of lung cancer cells in nude mice by inhibiting EGFR/MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yingqiu Yin,Junling Xie,Feng Peng,Liming Tan,Yun Xiao,Huiying Zheng,Lingzhi Yin,Huijing Situ,Shanqiang Zhang

Mediators of inflammation 2022:1108569 PubMed36619207

2022

Prevents Ulcerative Colitis by Promoting M2 Macrophage Polarization through Blocking the TLR4/MAPK/NF-B Pathway.

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Species

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Jun Sun,Ping Zhao,Xufeng Ding,Fang Li,Jie Jiang,Hua Huang,Lijiang Ji

Frontiers in pharmacology 12:791889 PubMed35173610

2022

LncRNA LINC01134 Contributes to Radioresistance in Hepatocellular Carcinoma by Regulating DNA Damage Response MAPK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyi Wang,Xinxing Wang,Zhonghou Rong,Longfei Dai,Chengkun Qin,Shikang Wang,Wenmao Geng

Pharmaceuticals (Basel, Switzerland) 15: PubMed35215358

2022

Design, Synthesis, and Biological Evaluation of a Novel VEGFR-2 Inhibitor Based on a 1,2,5-Oxadiazole-2-Oxide Scaffold with MAPK Signaling Pathway Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Mater H Mahnashi,Fardous F El-Senduny,Mohammed Abdulrahman Alshahrani,Mahrous A Abou-Salim

Molecular pain 17:17448069211003375 PubMed33745380

2021

Inhibition of the phosphoinositide 3-kinase-AKT-cyclic GMP-c-Jun N-terminal kinase signaling pathway attenuates the development of morphine tolerance in a mouse model of neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Travis Okerman,Taylor Jurgenson,Madelyn Moore,Amanda H Klein

Molecular medicine reports 21:1011-1020 PubMed31922242

2020

miR‑155 inhibition represents a potential valuable regulator in mitigating myocardial hypoxia/reoxygenation injury through targeting BAG5 and MAPK/JNK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xi,Qiang-Qiang Li,Bing-Qiang Li,Ning Li

Chemico-biological interactions 315:108907 PubMed31778667

2019

Treadmill exercise rescues mitochondrial function and motor behavior in the CAG knock-in mouse model of Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Charles C Caldwell,Giselle M Petzinger,Michael W Jakowec,Enrique Cadenas

International journal of biological sciences 15:493-506 PubMed30745837

2019

CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Guoqin Zhu,Naixin Jia,Weiyi Wang,Yuan Wang,Meifang Yin,Xuping Jiang,Yuesheng Huang,Jiaping Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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JNK 1/2 (pT183/Y185 + Total) ELISA Kit

ELISA - JNK 1/2 (pT183/Y185 + Total) ELISA Kit (AB176662)

Sandwich ELISA - JNK 1/2 (pT183/Y185 + Total) ELISA Kit (AB176662)

Sandwich ELISA - JNK 1/2 (pT183/Y185 + Total) ELISA Kit (AB176662)

Sandwich ELISA - JNK 1/2 (pT183/Y185 + Total) ELISA Kit (AB176662)

Sandwich ELISA - JNK 1/2 (pT183/Y185 + Total) ELISA Kit (AB176662)

Sandwich ELISA - JNK 1/2 (pT183/Y185 + Total) ELISA Kit (AB176662)

Key facts

Detection method

Sample types

Reacts with

Assay type

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise