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AB318950

Kanamycin ELISA Kit - Extracellular

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Kanamycin ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Kanamycin with a sensitivity of 38.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
5 Images
Sandwich ELISA - Kanamycin ELISA Kit - Extracellular (AB318950)
  • sELISA

Supplier Data

Sandwich ELISA - Kanamycin ELISA Kit - Extracellular (AB318950)

Example of Kanamycin standard curve in Sample Diluent NS. Example of Kanamycin standard curve. OD (450 nm) values (mean +/- SD) are graphed.

Competitive ELISA - Kanamycin ELISA Kit - Extracellular (AB318950)
  • cELISA

Supplier Data

Competitive ELISA - Kanamycin ELISA Kit - Extracellular (AB318950)

Interpolated concentrations of Kanamycin in Human urine, Bovine milk, and cell culture media with and without 10% FBS. Kanamycin was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are as follows : 20% Human Urine, 10% Bovine Milk, and 50% cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Competitive ELISA - Kanamycin ELISA Kit - Extracellular (AB318950)
  • cELISA

Supplier Data

Competitive ELISA - Kanamycin ELISA Kit - Extracellular (AB318950)

Interpolated concentrations of spiked Kanamycin in Mouse serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Kanamycin was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 20% Mouse serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Competitive ELISA - Kanamycin ELISA Kit - Extracellular (AB318950)
  • cELISA

Supplier Data

Competitive ELISA - Kanamycin ELISA Kit - Extracellular (AB318950)

Interpolated concentrations of spiked Kanamycin in Human serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Kanamycin was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 20% Human serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Competitive ELISA - Kanamycin ELISA Kit - Extracellular (AB318950)
  • cELISA

Supplier Data

Competitive ELISA - Kanamycin ELISA Kit - Extracellular (AB318950)

Example of Kanamycin standard curve. OD (450 nm) values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Urine, Cell culture media, Milk

Assay type

Competitive

Sensitivity

= 38.3 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Kanamycin SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of Kanamycin in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Urine, Cell culture media, and Milk. Quantitate Kanamycin with 38.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture conjugates conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA allows the formation of the antibody-analyte complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Milk", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.3" }, { "reproducibilityType": "Inter", "sample": "Milk", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.8" } ]
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Recovery

[ { "sample": "Serum", "range": "103 - 107 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "101 - 110 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "110 - 111 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "101 - 107 %", "average": "= 104" }, { "sample": "Urine", "range": "94 - 117 %", "average": "= 102" }, { "sample": "Cell culture media", "range": "83 - 97 %", "average": "= 91" }, { "sample": "Milk", "range": "102 - 119 %", "average": "= 108" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab318950-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB318950-CMP08" }, { "size":"1 x 100 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318950-CMP06" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB318950-CMP07" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318950-CMP05" }, { "size":"10 x 8 mL", "name":"Antibody Diluent 4BR", "number":"AB318950-CMP04" }, { "size":"10 x 2 Vial", "name":"Kanamycin Lyophilized Standard", "number":"AB318950-CMP03" }, { "size":"10 x 80 µL", "name":"50X Kanamycin CaptSure Conjugate", "number":"AB318950-CMP01" }, { "size":"10 x 80 µL", "name":"50X Kanamycin HRP Conjugate", "number":"AB318950-CMP02" }, { "size":"10 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318950-CMP09" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB318950-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab318950-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB318950-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318950-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB318950-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318950-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Antibody Diluent 4BR", "number":"AB318950-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Kanamycin Lyophilized Standard", "number":"AB318950-CMP03", "productcode":"" }, { "size":"1 x 80 µL", "name":"50X Kanamycin CaptSure Conjugate", "number":"AB318950-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"50X Kanamycin HRP Conjugate", "number":"AB318950-CMP02", "productcode":"" }, { "size":"1 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318950-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB318950-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information Kanamycin
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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