JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB318951

Kanamycin ELISA Kit - Intracellular

Be the first to review this product! Submit a review

|

(0 Publication)

Kanamycin ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify Kanamycin with a sensitivity of 33.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
3 Images
Competitive ELISA - Kanamycin ELISA Kit - Intracellular (AB318951)
  • cELISA

Supplier Data

Competitive ELISA - Kanamycin ELISA Kit - Intracellular (AB318951)

Interpolated concentrations of spiked ​Kanamycin​ in chicken, pork, and shrimp extracts. ​​Kanamycin​ was spiked into the following samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. Undiluted samples are 1 mg/mL chicken extract, pork extract, and shrimp extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Competitive ELISA - Kanamycin ELISA Kit - Intracellular (AB318951)
  • cELISA

Supplier Data

Competitive ELISA - Kanamycin ELISA Kit - Intracellular (AB318951)

Interpolated concentrations of spiked ​Kanamycin​ in mouse kidney extract. ​​Kanamycin​ was spiked into the following sample and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. Undiluted sample is 0.25 mg/mL mouse kidney extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Competitive ELISA - Kanamycin ELISA Kit - Intracellular (AB318951)
  • cELISA

Supplier Data

Competitive ELISA - Kanamycin ELISA Kit - Intracellular (AB318951)

Example of ​Kanamycin​ standard curve. OD (450 nm) values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Assay type

Competitive

Sensitivity

= 33.7 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Product details

Kanamycin SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of Kanamycin in Tissue Extracts. Quantitate Kanamycin with 33.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture conjugates conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA allows the formation of the antibody-analyte complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Tissue Extracts", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.3" }, { "reproducibilityType": "Inter", "sample": "Tissue Extracts", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.5" } ]
style
left-column

Recovery

[ { "sample": "Tissue Extracts", "range": "96 - 108 %", "average": "= 103" } ]
style
left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab318951-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB318951-CMP08" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB318951-CMP07" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB318951-CMP11" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318951-CMP05" }, { "size":"10 x 8 mL", "name":"Antibody Diluent 4BR", "number":"AB318951-CMP04" }, { "size":"10 x 2 Vial", "name":"Kanamycin Lyophilized Standard", "number":"AB318951-CMP03" }, { "size":"10 x 80 µL", "name":"50X Kanamycin CaptSure Conjugate", "number":"AB318951-CMP01" }, { "size":"10 x 80 µL", "name":"50X Kanamycin HRP Conjugate", "number":"AB318951-CMP02" }, { "size":"10 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318951-CMP09" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB318951-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab318951-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB318951-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318951-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB318951-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Antibody Diluent 4BR", "number":"AB318951-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318951-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Kanamycin Lyophilized Standard", "number":"AB318951-CMP03", "productcode":"" }, { "size":"1 x 80 µL", "name":"50X Kanamycin CaptSure Conjugate", "number":"AB318951-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"50X Kanamycin HRP Conjugate", "number":"AB318951-CMP02", "productcode":"" }, { "size":"1 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318951-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB318951-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB318951-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

See full target information Kanamycin
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
websiteProtocolBooklet
en
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com