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AB133040

Leukotriene B4 ELISA Kit

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(6 Publications)

Leukotriene B4 ELISA Kit is a Competitive ELISA for the measurement of Leukotriene B4 in Human, Pig in Biofluids, Cell Culture Media samples.

View Alternative Names

Leukotriene B4

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Competitive ELISA - Leukotriene B4 ELISA Kit (AB133040)
  • cELISA

Supplier Data

Competitive ELISA - Leukotriene B4 ELISA Kit (AB133040)

Representative Standard Curve using ab133040

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Plasma, Cell culture supernatant

Reacts with

Human, Pig

Assay type

Competitive

Sensitivity

= 5.63 pg/mL

Range

11.7 - 3000 pg/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Leukotriene B4 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Leukotriene B4 (LTB4) in tissue culture media, plasma, urine and saliva.

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-LTB4 antigen and a polyclonal rabbit antibody specific to LTB4. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of LTB4 captured in the plate.

Leukotrienes are major products of 5-lipoxygenase metabolism of arachidonic acid. Leukotriene B4 (LTB4) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation. The cysteinyl leukotrienes (LTC4, LTD4, and LTE4) are formed by addition of cysteine derivatives to LTA4. The conversion of LTA4 to LTC4 by the enzyme LTC4 synthase limits the rate of cysteinyl leukotriene formation. Cysteinyl leukotrienes are potent lipid mediators in inflammation, inducing smooth muscle contractions and increased capillary permeability.

Cross Reactivity

Compound Cross Reactivity %
LTB4 100
6-trans-12-epi-LTB4 5.50
12-epi-LTB4 4.90
PGE2 0.94
PGF2α <0.2
20-OH-LTB4 <0.2
20-COOH-LTB4 <0.2
LTC4 <0.2
LTD4 <0.2
LTE4 <0.2
5(S)-HETE <0.2
12(S)-HETE <0.2
15(S)-HETE <0.2
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Precision

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 0, "mean": "507 pg/mL", "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Inter", "sample": "Low", "replicates": 0, "mean": "99 pg/mL", "standardDeviation": null, "coefficientOfVariability": "15.7" }, { "reproducibilityType": "Inter", "sample": "Media", "replicates": 0, "mean": "308 pg/mL", "standardDeviation": null, "coefficientOfVariability": "16.5" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 0, "mean": "1078 pg/mL", "standardDeviation": null, "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Intra", "sample": "Low", "replicates": 0, "mean": "305 pg/mL", "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Media", "replicates": 0, "mean": "607 pg/mL", "standardDeviation": null, "coefficientOfVariability": "6.8" } ]
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Recovery

[ { "sample": "Plasma", "range": null, "average": "= 109.6" }, { "sample": "Urine", "range": null, "average": "= 96.9" }, { "sample": "Saliva", "range": null, "average": "= 114.1" }, { "sample": "Tissue Culture Media", "range": null, "average": "= 97.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab133040-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Sealer", "number":"AB133040-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Goat anti-rabbit IgG Microplate (12 x 8 wells)", "number":"AB133040-CMP09", "productcode":"" }, { "size":"1 x 27 mL", "name":"20X Wash Buffer Concentrate", "number":"AB133040-CMP08", "productcode":"" }, { "size":"1 x 5 mL", "name":"Leukotriene B4 Antibody", "number":"AB133040-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"Leukotriene B4 Alkaline Phosphatase Conjugate", "number":"AB133040-CMP04", "productcode":"" }, { "size":"1 x 500 µL", "name":"Leukotriene B4 Standard", "number":"AB133040-CMP02", "productcode":"" }, { "size":"1 x 27 mL", "name":"Assay Buffer", "number":"AB133040-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"pNpp Substrate", "number":"AB133040-CMP06", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB133040-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Leukotriene B4 commonly referred to as LTB4 is a bioactive lipid molecule with an approximate molecular mass of 279.45 g/mol. It is a part of the leukotriene family which are eicosanoids synthesized from arachidonic acid via the 5-lipoxygenase (5-LO) pathway. LTB4 is primarily expressed by leukocytes such as neutrophils as well as macrophages and mast cells. Its production occurs mostly in response to inflammatory stimuli which makes it an important mediator in immune responses.
Biological function summary

LTB4 acts as a potent chemoattractant and activator of leukocytes mediating immune cell recruitment to sites of inflammation. It binds specifically to G-protein-coupled receptors BLT1 and BLT2 on the surface of target cells leading to activation and subsequent response. LTB4 is not part of a larger protein complex; instead it functions as a standalone signaling lipid. Through its interaction with BLT receptors it plays a role in amplifying inflammatory signals contributing to the body's defense mechanisms.

Pathways

LTB4 is deeply embedded in the arachidonic acid metabolic pathway which includes the lipoxygenase and cyclooxygenase paths. This pathway is essential for the production of other eicosanoids involved in inflammation and immunity such as prostaglandins. LTB4 works alongside other leukotrienes in modulating the inflammatory response. It is closely related to proteins like 5-lipoxygenase which catalyzes the initial steps leading to its synthesis and also interacts with protein kinase pathways that mediate cellular responses.

LTB4 has significant relevance in chronic inflammatory conditions like asthma and rheumatoid arthritis. In asthma it contributes to bronchoconstriction and leukocyte infiltration which worsen the disease symptoms. Rheumatoid arthritis sees LTB4 facilitating the infiltration and activation of immune cells within joints thereby exacerbating inflammation and tissue damage. It connects to proteins like interleukin-8 (IL-8) in these contexts which further propagate inflammatory signals highlighting LTB4's critical role in the pathology of inflammatory diseases.
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Product protocols

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Target data

See full target information LTB4
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36613462

2022

Iron Dyshomeostasis in COVID-19: Biomarkers Reveal a Functional Link to 5-Lipoxygenase Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Beatrice Dufrusine,Silvia Valentinuzzi,Sandra Bibbò,Verena Damiani,Paola Lanuti,Damiana Pieragostino,Piero Del Boccio,Ersilia D'Alessandro,Alberto Rabottini,Alessandro Berghella,Nerino Allocati,Katia Falasca,Claudio Ucciferri,Francesco Mucedola,Marco Di Perna,Laura Martino,Jacopo Vecchiet,Vincenzo De Laurenzi,Enrico Dainese

American journal of translational research 13:13815-13824 PubMed35035721

2021

Efficacy of tiotropium bromide combined with different doses of fluticasone plus salmeterol DPI in the treatment of stable COPD.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Ma,Jingjing Xu,Jun Yang

International journal of chronic obstructive pulmonary disease 15:2379-2388 PubMed33061355

2020

Serum Amyloid A in Stable COPD Patients is Associated with the Frequent Exacerbator Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Dongxing Zhao,Asghar Abbasi,Harry B Rossiter,Xiaofen Su,Heng Liu,Yuhong Pi,Li Sang,Weiyong Zhong,Qifeng Yang,Xiongtian Guo,Yanyan Zhou,Tianyang Li,Richard Casaburi,Nuofu Zhang

Prostaglandins, leukotrienes, and essential fatty acids 127:25-31 PubMed29156155

2017

Leukotriene B4 indicates lung injury and on-going inflammatory changes after severe trauma in a porcine long-term model.

Applications

ELISA

Species

Unspecified reactive species

Philipp Störmann,Birgit Auner,Lukas Schimunek,Rafael Serve,Klemens Horst,Tim-P Simon,Roman Pfeifer,Kernt Köhler,Frank Hildebrand,Sebastian Wutzler,Hans-Christoph Pape,Ingo Marzi,Borna Relja

Experimental and therapeutic medicine 14:2925-2930 PubMed28912850

2017

Effects of leukotriene B4 on interleukin-32, interferon-γ and chemokines in rats with rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Danyan Bi,Danqing Bi,Ming Zhong,Hong Zhang,Song Jin,Sha Ma,Huayou Luo

PloS one 9:e90122 PubMed24587235

2014

Modulation of basophils' degranulation and allergy-related enzymes by monomeric and dimeric naphthoquinones.

Applications

Unspecified application

Species

Unspecified reactive species

Brígida R Pinho,Carla Sousa,Patrícia Valentão,Jorge M A Oliveira,Paula B Andrade
View all publications
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