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AB285324

Leukotriene B4 ELISA Kit

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Leukotriene B4 ELISA Kit is a ELISA for the measurement of Leukotriene B4 in in Biofluids samples.

View Alternative Names

BLT, BLT1, BLTR, CMKRL1, GPR16, P2RY7, LTB4R, Leukotriene B4 receptor 1, LTB4-R 1, LTB4-R1, Chemoattractant receptor-like 1, G-protein coupled receptor 16, P2Y purinoceptor 7, P2Y7

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ELISA - Leukotriene B4 ELISA Kit (AB285324)
  • ELISA

Supplier Data

ELISA - Leukotriene B4 ELISA Kit (AB285324)

Standard curve for Leukotriene B4 ELISA Kit ab285324

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Sensitivity

= 9.38 pg/mL

Range

15.63 - 1000 pg/mL

Assay Platform

Microplate (12 x 8 well strips)

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Leukotriene B4 ELISA Kit (ab285324, E4793) is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with LTB4. During the reaction, LTB4 in the samples or standard competes with LTB4 coated on the plate for binding to the anti-LTB4 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of LTB4. The concentration of LTB4 in the samples can be calculated by comparing the OD of the samples to the standard curve.

This kit recognizes LTB4 in samples. No Significant cross-reactivity or interference between LTB4 and analogues was observed.

This product is manufactured by BioVision, an Abcam company and was previously called E4793 Leukotriene B4 ELISA Kit. E4793-100 is the same size as the 96 test size of ab285324.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 20, "mean": "50.4 pg/mL", "standardDeviation": "2.8", "coefficientOfVariability": "5.56" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 20, "mean": "116.6 pg/mL", "standardDeviation": "4.8", "coefficientOfVariability": "4.12" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 20, "mean": "318.6 pg/mL", "standardDeviation": "15.3", "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 20, "mean": "54.3 pg/mL", "standardDeviation": "3.5", "coefficientOfVariability": "6.45" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 20, "mean": "119.2 pg/mL", "standardDeviation": "5", "coefficientOfVariability": "4.19" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 20, "mean": "336.5 pg/mL", "standardDeviation": "18.2", "coefficientOfVariability": "5.41" } ]
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Recovery

[ { "sample": "Serum", "range": "89 - 105 %", "average": "= 96" }, { "sample": "Cell culture media", "range": "93 - 106 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "86 - 98 %", "average": "= 91" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285324-96Test", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Reference Sample and Standard Diluent", "number":"AB285324-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X Biotinylated Detection Antibody", "number":"AB285324-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285324-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Reference Standard", "number":"AB285324-CMP07", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X HRP-Conjugate", "number":"AB285324-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"Substrate Reagent", "number":"AB285324-CMP10", "productcode":"" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285324-CMP11", "productcode":"" }, { "size":"1 x 14 mL", "name":"HRP-Conjugate Diluent", "number":"AB285324-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Sealer", "number":"AB285324-CMP05", "productcode":"" }, { "size":"1 x 14 mL", "name":"Biotinylated Detection Antibody Diluent", "number":"AB285324-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285324-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Leukotriene B4 (LTB4) is a potent lipid mediator and part of the leukotriene family derived from arachidonic acid by 5-lipoxygenase. It has a molecular mass of approximately 336.5 Da. LTB4 is primarily produced by leukocytes including neutrophils macrophages and mast cells. It plays an important role in inflammatory processes by acting as a chemoattractant for neutrophils guiding them to sites of tissue damage or infection.
Biological function summary

LTB4 functions in immune response regulation by activating leukocytes through binding to its specific G-protein coupled receptor known as BLT1. This interaction leads to a series of intracellular signaling events that result in degranulation superoxide production and the release of pro-inflammatory cytokines. The actions of LTB4 are not typically part of a larger multimeric complex; instead its activity involves direct interactions with cell surface receptors illustrating its role as a messenger in inflammatory response.

Pathways

The biosynthesis of LTB4 is a component of the arachidonic acid metabolism pathway. It forms through the lipoxygenase pathway and is closely related to other eicosanoids like leukotriene A4. The relation with proteins such as 5-lipoxygenase-activating protein (FLAP) is significant as this protein facilitates the conversion of arachidonic acid into leukotrienes anchoring LTB4 within the broader framework of inflammatory mediators and signaling molecules.

LTB4 plays a significant role in conditions such as asthma and rheumatoid arthritis. Elevated levels of LTB4 are often observed in the inflamed airways of asthma patients correlating with bronchoconstriction and airway hyperreactivity. Similarly in rheumatoid arthritis LTB4 contributes to joint inflammation and pain. In these contexts LTB4 interacts with proteins like cysteinyl leukotrienes contributing to the severity of inflammatory manifestations and offering a target for therapeutic intervention.
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Product protocols

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Target data

Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response.
See full target information LTB4R
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Product promise

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