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AB136942

LVV Hemorphin 7 ELISA kit

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LVV Hemorphin 7 ELISA kit is a Competitive ELISA for the measurement of LVV Hemorphin 7 in Human, Rat in Cell/Tissue Extracts, Biofluids samples.

View Alternative Names

Hemoglobin subunit beta, Beta-globin, Hemoglobin beta chain, HBB

2 Images
Competitive ELISA - LVV Hemorphin 7 ELISA kit (AB136942)
  • cELISA

Supplier Data

Competitive ELISA - LVV Hemorphin 7 ELISA kit (AB136942)

Typical Parallelism Graph.

Dose‐response curves from human plasma and serum diluted into assay buffer were compared to the LVV Hemorphin 7 standard curve. The parallel response indicates the standard effectively mimics the native protein.

Competitive ELISA - LVV Hemorphin 7 ELISA kit (AB136942)
  • cELISA

Supplier Data

Competitive ELISA - LVV Hemorphin 7 ELISA kit (AB136942)

Representative Standard Curve using ab136942.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Serum

Reacts with

Rat, Human

Assay type

Competitive

Sensitivity

= 6.1 pg/mL

Range

9.8 - 10000 pg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's LVV Hemorphin 7 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of LVV Hemorphin 7 in serum and tissue homogenates. This kit it not intended for use with plasma samples.

A polyclonal anti-Rabbit-IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a solution of LVV Hemorphin 7 conjugated to biotin, followed by a solution of polyclonal antibody to LVV Hemorphin 7. The plate is washed to remove unbound reagents. A solution of streptavidin-HRP conjugate is then added. After further incubation the excess reagents are washed away and TMB substrate is added, which is catalyzed by HRP to generate a yellow color. A stop solution changes this color from yellow to blue, and the intensity of this blue coloration is inversely proportional to the amount of LVV Hemorphin 7 captured in the plate.

Analyte Sequence Percent cross reactivities in the range of 0.1 pM ‐ 500 nM
LVV Hemorphin 7 LVVYPWTQRF 100
Leu-Valorphin-Arg (LVV Hemorphin-6) VVYPWTQR <0.003
Valorphin VVYPWTQ <0.003
Ang(1-12) DRVYIHPFHLVI <0.003
Ang I DRVYIHPFHL <0.003
Ang(1-9) DRVYIHPFH <0.003
Ang II DRVYIHPF <0.003
Ang(1-7) DRVYIHP <0.003
Ang A ARVYIHPF <0.003
Ang III RVYIHPF <0.003
Ang IV VYIHPF <0.003
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Precision

[ { "reproducibilityType": "Inter", "sample": "LVV Hem 7", "replicates": 0, "mean": "334.1 pg/mL", "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Inter", "sample": "LVV Hem 7", "replicates": 0, "mean": "707 pg/mL", "standardDeviation": null, "coefficientOfVariability": "9.2" }, { "reproducibilityType": "Inter", "sample": "LVV Hem 7", "replicates": 0, "mean": "72.3 pg/mL", "standardDeviation": null, "coefficientOfVariability": "16.5" }, { "reproducibilityType": "Intra", "sample": "LVV Hem 7", "replicates": 20, "mean": "708.1 pg/mL", "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "LVV Hem 7", "replicates": 20, "mean": "305.5 pg/mL", "standardDeviation": null, "coefficientOfVariability": "8.8" }, { "reproducibilityType": "Intra", "sample": "LVV Hem 7", "replicates": 20, "mean": "71.5 pg/mL", "standardDeviation": null, "coefficientOfVariability": "20.9" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hemoglobin A1c also known as HbA1c or glycohemoglobin is a form of hemoglobin that is chemically linked to a glucose molecule. It has a molecular weight of approximately 64 kDa. This protein is primarily found in red blood cells where it carries oxygen from the lungs to the rest of the body. Hemoglobin A1c is produced when glucose in the blood binds irreversibly to hemoglobin a process known as glycation. Researchers measure Hemoglobin A1c levels to manage blood sugar control particularly in diabetic patients.
Biological function summary

Hemoglobin A1c is a marker for assessing long-term glycemic control. It does not operate as part of a larger complex but modifies the hemoglobin protein inside erythrocytes. The level of HbA1c reflects the average blood glucose concentration over the previous two to three months. Its presence and levels offer insight into the overall metabolic state providing important information for clinicians while managing conditions like diabetes. HbA1c is important since it allows monitoring without the need for daily blood sugar measurements.

Pathways

HbA1c integrates into the glucose metabolism pathway and is linked to protein glycation pathways. Within the glucose metabolism pathway glycated hemoglobin serves as a longitudinal biomarker. It does not directly interact with other proteins like insulin but insulin levels can indirectly influence the HbA1c levels by regulating blood glucose levels. The accurate measurement of HbA1c by HbA1c assay or HbA1c ELISA provides insights into the efficacy of diabetes management strategies.

Hemoglobin A1c plays a significant role in diabetes mellitus and cardiovascular disease. High levels of HbA1c indicate poor glycemic control which is associated with complications of diabetes such as retinopathy. In cardiovascular disease elevated HbA1c levels act as a risk factor for adverse outcomes. The relationship between HbA1c and these diseases also highlights connections with other proteins such as insulin which regulates glucose and subsequently HbA1c levels. Utilizing HA1c test kits and HbA1c kits healthcare providers can monitor potential long-term complications in patients with elevated HbA1c levels.
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Product protocols

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Target data

Involved in oxygen transport from the lung to the various peripheral tissues.. LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure.. Spinorphin. Functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.
See full target information HBB
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websiteProtocolBooklet
en
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