JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325452

mCherry ELISA Kit, Chemiluminescent

Be the first to review this product! Submit a review

|

(0 Publication)

mCherry ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of mCherry with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :0.91 - 2000 pg/mL with a sensitivity of 0.42 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent Pending
7 Images
Sandwich ELISA - mCherry ELISA Kit, Chemiluminescent (AB325452)
  • sELISA

Lab

Sandwich ELISA - mCherry ELISA Kit, Chemiluminescent (AB325452)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - mCherry ELISA Kit, Chemiluminescent (AB325452)
  • sELISA

Lab

Sandwich ELISA - mCherry ELISA Kit, Chemiluminescent (AB325452)

Recombinant mCherry was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 100% RPMI cell culture media + 10% FBS and 100% HUVEC supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - mCherry ELISA Kit, Chemiluminescent (AB325452)
  • sELISA

Lab

Sandwich ELISA - mCherry ELISA Kit, Chemiluminescent (AB325452)

Recombinant mCherry was spiked into the following samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. Undiluted samples are 200 µg/mL HEK293T cell extract and 143B (wild type) cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - mCherry ELISA Kit, Chemiluminescent (AB325452)
  • sELISA

Lab

Sandwich ELISA - mCherry ELISA Kit, Chemiluminescent (AB325452)

Dynamic range comparison between the Chemiluminescent mCherry SimpleStep ELISA and colorimetric mCherry SimpleStep ELISA (ab221829). The Chemiluminescent SimpleStep ELISA kit shows increased dynamic range and sensitivity.

Sandwich ELISA - mCherry ELISA Kit, Chemiluminescent (AB325452)
  • sELISA

Lab

Sandwich ELISA - mCherry ELISA Kit, Chemiluminescent (AB325452)

Raw O.D. values are shown for mCherry protein, YFP, GFP, and RFP. All proteins were loaded at 400 pg/mL. The average background O.D. is represented by the dashed line. Signal from YFP, GFP, and RFP loaded at 400 pg/mL was equivalent to background.

Sandwich ELISA - mCherry ELISA Kit, Chemiluminescent (AB325452)
  • sELISA

Lab

Sandwich ELISA - mCherry ELISA Kit, Chemiluminescent (AB325452)

Standard curve comparison between the Chemiluminescent mCherry SimpleStep ELISA and colorimetric mCherry SimpleStep ELISA (ab221829) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - mCherry ELISA Kit, Chemiluminescent (AB325452)
  • sELISA

Lab

Sandwich ELISA - mCherry ELISA Kit, Chemiluminescent (AB325452)

Standard curve comparison between the Chemiluminescent mCherry SimpleStep ELISA and colorimetric mCherry SimpleStep ELISA (ab221829) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Cell Extract

Assay type

Sandwich (quantitative)

Sensitivity

= 0.42 pg/mL

Range

0.91 - 2000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

mCherry ELISA Kit, Chemiluminescent (ab325452) is a rapid single-wash sandwich 55-min ELISA to measure mCherry in Cell Culture Supernatant, Cell Extract with a sensitivity of 0.42 pg/ml

How the assay works

mCherry ELISA Kit, Chemiluminescent (ab325452) SimpleStep ELISA®  employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.<br>Please refer to our protocol booklet for more details.

mCherry (ab325452) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-mCherry antibody [EPR20579-246] - BSA and Azide free (Capture) ab244649

- Anti-mCherry antibody [EPR20579-1] - BSA and Azide free (Detector) ab244910

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.9" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.7" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "110 - 113 %", "average": "= 111" }, { "sample": "Cell Lysate", "range": "97 - 102 %", "average": "= 100" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325452-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent 4BC", "number":"AB325452-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X mCherry Capture Antibody", "number":"AB325452-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325452-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325452-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB325452-CMP05", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325452-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB325452-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"mCherry Lyophilized Recombinant Protein", "number":"AB325452-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X mCherry Detector Antibody", "number":"AB325452-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325452-CMP12", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325452-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325452-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

See full target information mCherry
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com