AB126448

Mek1 (pS217/221) + total Mek1 ELISA Kit

Name: Mek1 (pS217/221) + total Mek1 ELISA Kit (ab126448) | Abcam
Brand: Abcam Limited
SKU: AB126448

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Mek1 (pS217/221) + total Mek1 ELISA Kit is a Semi-quantitative ELISA for the measurement of Mek1 (pS217/221) + total Mek1 in Human, Mouse, Rat in Cell/Tissue Extracts samples.

View Alternative Names

MEK1, PRKMK1, MAP2K1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1

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Sandwich ELISA - Mek1 (pS217/221) + total Mek1 ELISA Kit (AB126448)

sELISA

Supplier Data

Sandwich ELISA - Mek1 (pS217/221) + total Mek1 ELISA Kit (AB126448)

HeLa cells were treated or untreated with TPA for 15 min. Cell lysates were analyzed using ab126448.

sELISA

Supplier Data

Sandwich ELISA - Mek1 (pS217/221) + total Mek1 ELISA Kit (AB126448)

HeLa cells were treated with TPA at 37°C for 15 min. Solubilize cells at 4 x 10⁷ cells/ml in Cell Lysate Buffer. Serial dilutions of cell lysates were analyzed using ab126448.

Supplier Data

Western blot - Mek1 (pS217/221) + total Mek1 ELISA Kit (AB126448)

HeLa cells were treated or untreated with TPA for 15 min. Cell lysates were analyzed by Western Blot.

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Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse, Rat, Human

Assay type

Semi-quantitative

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

ab126448 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated Mek1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-Mek1 (Ser217/221) and total Mek1 in human, mouse and rat cell lysates (help normalize the results of phospho-Mek1 from different cell lysate being compared). An anti-pan Mek1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Mek1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Mek1 (Ser217/221) or anti-pan-Mek1 antibody is used to detect phosphorylated Mek1 (Ser217/221) or total Mek1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mek1 (Ser217/221) or total Mek1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEK1 also known as MAP2K1 is a dual-specificity kinase that plays a role in the MAPK/ERK pathway. It has a molecular weight of around 45 kDa. MEK1 phosphorylates and activates the extracellular signal-regulated kinases ERK1 and ERK2. MEK1 is expressed in various tissues but is especially abundant in brain and heart tissues. Researchers commonly measure MEK1 levels using techniques like MEK1 ELISA while phospho-MEK antibodies help analyze its activity state.

Biological function summary

MEK1 impacts cell processes such as proliferation differentiation and survival. It does not work alone but forms a complex with other proteins to exert its function. MEK1 activity is tightly controlled by upstream activators and downstream targets. One of the key phosphorylation sites on MEK1 is at serine 298 often marked as MEK1 pS298. This phosphorylation indicates MEK1's activated state which is critical for its biological function.

Pathways

MEK1 integrates signals within the MAPK/ERK pathway and interacts closely with RAF kinases upstream and ERK kinases downstream. This pathway modulates cellular responses to growth factors. MEK1 undergoes phosphorylation enhancing its activity and subsequently phosphorylates ERK proteins. Many studies isolate MEK1 activity using specific inhibitors such as the MEK1 inhibitor to dissect pathway dynamics. ERK inhibitor PD98059 also helps when studying parallel interactions with MEK1.

MEK1 mutations or dysregulation have links to several types of cancer including melanoma. These often involve changes that lead to continuous MEK1 activation contributing to uncontrolled cell growth. MEK1 involvement in the RASopathies also shows its connection to disorders characterized by heart and facial abnormalities. In cancer MEK1 works closely with the BRAF protein which often harbors mutations leading to disease progression showcasing MEK1 as a target for therapeutic intervention.

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Product protocols

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Target data

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed : 29433126). Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

See full target information MAP2K1 phospho S217 + S221

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mek1 (pS217/221) + total Mek1 ELISA Kit

Sandwich ELISA - Mek1 (pS217/221) + total Mek1 ELISA Kit (AB126448)

Sandwich ELISA - Mek1 (pS217/221) + total Mek1 ELISA Kit (AB126448)

Western blot - Mek1 (pS217/221) + total Mek1 ELISA Kit (AB126448)

Key facts

Detection method

Sample types

Reacts with

Assay type

Assay time

Assay Platform

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise