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AB283259

Melatonin ELISA kit

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(1 Publication)

Melatonin ELISA kit is a competitive ELISA designed to quantify Melatonin with a sensitivity of 2.5 pg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 3 - 300 pg/ml

View Alternative Names

5 methoxy N acetyltryptamine, N acetyl 5 methoxytryptamine

Key facts

Detection method

Colorimetric

Sample types

EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

= 2.5 pg/mL

Range

3 - 300 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Melatonin ELISA kit (ab283259) is based on the competition principal and microplate separation. Melatonin in calibrators and samples compete with a fixed amount of melatonin conjugated to horse radish peroxidase (Melatonin-HRP) for binding sites with a rabbit melatonin monoclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm.

The amount of Melatonin-HRP detected is inversely proportional to the amount of melatonin in a sample.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 12, "mean": "270.1 pg/mL", "standardDeviation": "18.024", "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Inter", "sample": "Low", "replicates": 12, "mean": "21.8 pg/mL", "standardDeviation": "2.147", "coefficientOfVariability": "9.8" }, { "reproducibilityType": "Inter", "sample": "Media", "replicates": 12, "mean": "138.5 pg/mL", "standardDeviation": "7.254", "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 20, "mean": "270.6 pg/mL", "standardDeviation": "16.987", "coefficientOfVariability": "6.3" }, { "reproducibilityType": "Intra", "sample": "Low", "replicates": 20, "mean": "23.1 pg/mL", "standardDeviation": "2.2", "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Intra", "sample": "Media", "replicates": 20, "mean": "141.6 pg/mL", "standardDeviation": "8.614", "coefficientOfVariability": "6.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283259-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Antibody Coated Wells 96 wells plate", "number":"AB283259-CMP13", "productcode":"" }, { "size":"1 x 500 µL", "name":"Melatonin Standard 6", "number":"AB283259-CMP09", "productcode":"" }, { "size":"1 x 500 µL", "name":"Melatonin Standard 1", "number":"AB283259-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Wash Solution Concentrated (10X)", "number":"AB283259-CMP12", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Reagent", "number":"AB283259-CMP11", "productcode":"" }, { "size":"1 x 500 µL", "name":"Melatonin Standard 5", "number":"AB283259-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB283259-CMP10", "productcode":"" }, { "size":"1 x 500 µL", "name":"Melatonin Standard 3", "number":"AB283259-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Anti-Melatonin Solution", "number":"AB283259-CMP14", "productcode":"" }, { "size":"1 x 500 µL", "name":"Control 1", "number":"AB283259-CMP01", "productcode":"" }, { "size":"1 x 500 µL", "name":"Melatonin Standard 2", "number":"AB283259-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Enzyme Conjugate", "number":"AB283259-CMP03", "productcode":"" }, { "size":"1 x 500 µL", "name":"Melatonin Standard 4", "number":"AB283259-CMP07", "productcode":"" }, { "size":"1 x 500 µL", "name":"Control 2", "number":"AB283259-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information Melatonin
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of pineal research 73:e12821 PubMed35941749

2022

Chronic developmental hypoxia alters mitochondrial oxidative capacity and reactive oxygen species production in the fetal rat heart in a sex-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Kerri L M Smith,Agnieszka Swiderska,Mitchell C Lock,Lucia Graham,Wulan Iswari,Tashi Choudhary,Donna Thomas,Hager M Kowash,Michelle Desforges,Elizabeth C Cottrell,Andrew W Trafford,Dino A Giussani,Gina L J Galli
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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