Skip to main content

Melatonin ELISA kit is a Competitive ELISA kit for the measurement of Melatonin in Human in EDTA Plasma, Heparin Plasma, Serum samples.

Be the first to review this product! Submit a review

Publications

Key facts

Detection method
Colorimetric
Sample types
EDTA Plasma, Heparin Plasma, Serum
Assay type
Competitive
Reactive species
Human
Range
3 - 300 pg/mL
Sensitivity
= 2.5 pg/mL

Reactivity data

Application
cELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Alternative names

5 methoxy N acetyltryptamine, N acetyl 5 methoxytryptamine

What's included?

1 x 96 Tests
Components
Anti-Melatonin Solution
1 x 6 mL
Antibody Coated Wells 96 wells plate
1 x 1 Unit
Control 1
1 x 500 µL
Control 2
1 x 500 µL
Enzyme Conjugate
1 x 6 mL
Melatonin Standard 1
1 x 500 µL
Melatonin Standard 2
1 x 500 µL
Melatonin Standard 3
1 x 500 µL
Melatonin Standard 4
1 x 500 µL
Melatonin Standard 5
1 x 500 µL
Melatonin Standard 6
1 x 500 µL
Stop Solution
1 x 15 mL
TMB Reagent
1 x 15 mL
Wash Solution Concentrated (10X)
1 x 50 mL

Recommended products

Melatonin ELISA kit is a Competitive ELISA kit for the measurement of Melatonin in Human in EDTA Plasma, Heparin Plasma, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
EDTA Plasma, Heparin Plasma, Serum
Assay type
Competitive
Reactive species
Human
Range
3 - 300 pg/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 2.5 pg/mL

Precision

Intra assay

Sample
High
n
20
mean
270.6 pg/mL
SD
16.987
C.V.
6.3
Sample
Low
n
20
mean
23.1 pg/mL
SD
2.2
C.V.
9.5
Sample
Media
n
20
mean
141.6 pg/mL
SD
8.614
C.V.
6.1

Inter assay

Sample
High
n
12
mean
270.1 pg/mL
SD
18.024
C.V.
6.7
Sample
Low
n
12
mean
21.8 pg/mL
SD
2.147
C.V.
9.8
Sample
Media
n
12
mean
138.5 pg/mL
SD
7.254
C.V.
5.2

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Melatonin ELISA kit (ab283259) is based on the competition principal and microplate separation. Melatonin in calibrators and samples compete with a fixed amount of melatonin conjugated to horse radish peroxidase (Melatonin-HRP) for binding sites with a rabbit melatonin monoclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm.

The amount of Melatonin-HRP detected is inversely proportional to the amount of melatonin in a sample.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

0 product images

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com