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AB283258

Melatonin ELISA kit (salivary)

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Melatonin ELISA kit (salivary) is a competitive ELISA designed to quantify salivary Melatonin with a sensitivity of 0.6 pg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 1 - 64 pg/ml

View Alternative Names

5 methoxy N acetyltryptamine, N acetyl 5 methoxytryptamine

Key facts

Detection method

Colorimetric

Sample types

Saliva

Reacts with

Human

Assay type

Competitive

Sensitivity

= 0.6 pg/mL

Range

1 - 64 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Melatonin ELISA kit (salivary) (ab283258) is based on the competition principal and microplate separation. Melatonin in standards (calibrators) and samples compete with a fixed amount of melatonin conjugated to horse radish peroxidase (Melatonin-HRP) for binding sites with a rabbit melatonin monoclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm.

The amount of Melatonin-HRP detected is inversely proportional to the amount of melatonin in a sample.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 12, "mean": "26.6 pg/mL", "standardDeviation": "2.194", "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Inter", "sample": "Low", "replicates": 12, "mean": "5.6 pg/mL", "standardDeviation": "0.62", "coefficientOfVariability": "11.1" }, { "reproducibilityType": "Inter", "sample": "Media", "replicates": 12, "mean": "9.9 pg/mL", "standardDeviation": "0.971", "coefficientOfVariability": "9.8" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 20, "mean": "27.6 pg/mL", "standardDeviation": "1.33", "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Intra", "sample": "Low", "replicates": 20, "mean": "5.5 pg/mL", "standardDeviation": "0.275", "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Media", "replicates": 20, "mean": "9.9 pg/mL", "standardDeviation": "0.507", "coefficientOfVariability": "5.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283258-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Antibody Coated Wells 96 wells plate", "number":"AB283258-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"Assay Diluent", "number":"AB283258-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"Anti-Melatonin Solution", "number":"AB283258-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Conjugate Buffer", "number":"AB283258-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB283258-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Wash Solution Concentrated (10X)", "number":"AB283258-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Reagent", "number":"AB283258-CMP06", "productcode":"" }, { "size":"1 x 700 µL", "name":"Enzyme Conjugate", "number":"AB283258-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"Concentrated Stock Melatonin Control", "number":"AB283258-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"Concentrated Stock Melatonin Calibrator", "number":"AB283258-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information Melatonin
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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