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Melatonin ELISA kit (salivary) is a Competitive ELISA kit for the measurement of Melatonin (salivary) in Human in Saliva samples.

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Key facts

Detection method
Colorimetric
Sample types
Saliva
Assay type
Competitive
Reactive species
Human
Range
1 - 64 pg/mL
Sensitivity
= 0.6 pg/mL

Reactivity data

Application
cELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Alternative names

5 methoxy N acetyltryptamine, N acetyl 5 methoxytryptamine

What's included?

1 x 96 Tests
Components
Anti-Melatonin Solution
1 x 3 mL
Antibody Coated Wells 96 wells plate
1 x 1 Unit
Assay Diluent
1 x 20 mL
Concentrated Stock Melatonin Calibrator
1 x 200 µL
Concentrated Stock Melatonin Control
1 x 200 µL
Conjugate Buffer
1 x 6 mL
Enzyme Conjugate
1 x 700 µL
Stop Solution
1 x 15 mL
TMB Reagent
1 x 15 mL
Wash Solution Concentrated (10X)
1 x 50 mL

Recommended products

Melatonin ELISA kit (salivary) is a Competitive ELISA kit for the measurement of Melatonin (salivary) in Human in Saliva samples.

Key facts

Detection method
Colorimetric
Sample types
Saliva
Assay type
Competitive
Reactive species
Human
Range
1 - 64 pg/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 0.6 pg/mL

Precision

Intra assay

Sample
High
n
20
mean
27.6 pg/mL
SD
1.33
C.V.
4.8
Sample
Low
n
20
mean
5.5 pg/mL
SD
0.275
C.V.
5
Sample
Media
n
20
mean
9.9 pg/mL
SD
0.507
C.V.
5.1

Inter assay

Sample
High
n
12
mean
26.6 pg/mL
SD
2.194
C.V.
8.3
Sample
Low
n
12
mean
5.6 pg/mL
SD
0.62
C.V.
11.1
Sample
Media
n
12
mean
9.9 pg/mL
SD
0.971
C.V.
9.8

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Melatonin ELISA kit (salivary) (ab283258) is based on the competition principal and microplate separation. Melatonin in standards (calibrators) and samples compete with a fixed amount of melatonin conjugated to horse radish peroxidase (Melatonin-HRP) for binding sites with a rabbit melatonin monoclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm.

The amount of Melatonin-HRP detected is inversely proportional to the amount of melatonin in a sample.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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