JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB126450

Met (pY1234/pY1235) ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Met (pY1234/pY1235) ELISA Kit is a ELISA for the measurement of Met (pY1234/pY1235) in Human, Mouse in Cell/Tissue Extracts samples.

View Alternative Names

Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, Tyrosine-protein kinase Met, SF receptor, MET

4 Images
Sandwich ELISA - Met (pY1234/pY1235) ELISA Kit (AB126450)
  • sELISA

Supplier Data

Sandwich ELISA - Met (pY1234/pY1235) ELISA Kit (AB126450)

A431 cells were treated or untreated with 50 ng/ml recombinant Human HGF for 5 min. Cell lysates were analyzed using ab126450.

Sandwich ELISA - Met (pY1234/pY1235) ELISA Kit (AB126450)
  • sELISA

Supplier Data

Sandwich ELISA - Met (pY1234/pY1235) ELISA Kit (AB126450)

H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126450.

Sandwich ELISA - Met (pY1234/pY1235) ELISA Kit (AB126450)
  • sELISA

Supplier Data

Sandwich ELISA - Met (pY1234/pY1235) ELISA Kit (AB126450)

H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126450.

Western blot - Met (pY1234/pY1235) ELISA Kit (AB126450)
  • WB

Supplier Data

Western blot - Met (pY1234/pY1235) ELISA Kit (AB126450)

H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed by Western blot.

false

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse, Human

Results type

Semi-Quantitative

Assay time

5h

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB126451

Met (pY1234/pY1235) + total Met ELISA Kit

Sandwich ELISA - Met (pY1234/pY1235) + total Met ELISA Kit (AB126451)

  • 0
  • 0
Primary Antibodies

AB5662

Anti-Met (c-Met) (phospho Y1230 + Y1234 + Y1235) antibody

  • 4
  • 7
Assay Kits

AB315043

Ms/Rt Synaptophysin ELISA Kit

SimpleStep

Sandwich ELISA - Ms/Rt Synaptophysin ELISA Kit (AB315043)

  • 0
  • 0
Assay Kits

AB210575

Mouse/Rat Cytochrome C ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (AB210575)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

ab126450 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in Human and Mouse cell lysates. By determining phosphorylated Met protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of Human and Mouse phospho-Met (Tyr1234/1235). An anti-pan Met antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Met present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Met (Tyr1234/1235) antibody is used to detect phosphorylated Met (Tyr1234/1235). After washing away unbound antibody, HRP-conjugated anti-Rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Met (Tyr1234/1235) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab126450-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB126450-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB126450-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Met Microplate (12 strips x 8 wells) coated with anti-pan Met antibody", "number":"AB126450-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB126450-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control: lyophilized powder from H1993 cell lysate", "number":"AB126450-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB126450-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB126450-CMP06", "productcode":"" }, { "size":"1 x 25 µL", "name":"1000X HRP-conjugated anti-rabbit IgG", "number":"AB126450-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Detection Antibody Met (Y1234/1235)", "number":"AB126450-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of neuronal precursors, angiogenesis and kidney formation. During skeletal muscle development, it is crucial for the migration of muscle progenitor cells and for the proliferation of secondary myoblasts (By similarity). In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Also promotes differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis (By similarity).. (Microbial infection) Acts as a receptor for Listeria monocytogenes internalin InlB, mediating entry of the pathogen into cells.
See full target information MET phospho Y1235
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com