Met (pY1234/pY1235) + total Met ELISA Kit
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(3 Publications)
- sELISA
Supplier Data
Sandwich ELISA - Met (pY1234/pY1235) + total Met ELISA Kit (AB126451)
H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126451.
- sELISA
Supplier Data
Sandwich ELISA - Met (pY1234/pY1235) + total Met ELISA Kit (AB126451)
H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126451.
- sELISA
Supplier Data
Sandwich ELISA - Met (pY1234/pY1235) + total Met ELISA Kit (AB126451)
A431 cells were treated or untreated with 50 ng/ml recombinant human HGF for 5 min. Cell lysates were analyzed using ab126451.
- WB
Supplier Data
Western blot - Met (pY1234/pY1235) + total Met ELISA Kit (AB126451)
H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed by Western blot.
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Product details
ab126451 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates. By determining phosphorylated Met protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-Met (Tyr1234/1235) and total Met in human, and mouse cell lysates (help normalize the results of phospho-Met from different cell lysate being compared). A pan Met antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Met present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phospho-Met (Tyr1234/1235) or anti-pan-Met is used to detect phosphorylated or total Met. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Met (Tyr1234/1235) or total Met bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
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Publications (3)
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Journal of translational medicine 21:530 PubMed37543570
2023
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Scientific reports 10:19020 PubMed33149187
2020
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Oncotarget 9:26466-26490 PubMed29899871
2018
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