AB190533

Monkey Albumin ELISA Kit

Name: Monkey Albumin ELISA Kit (ab190533) | Abcam
Brand: Abcam Limited
SKU: AB190533
Price: 780 USD
Availability: InStock

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Monkey Albumin ELISA Kit is a sandwich ELISA used to quantify Monkey Albumin with a sensitivity of 1.795 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range – quantifies 12.5 – 400 ng/mL

View Alternative Names

Qlv-U347A-C12, Qlv-U377A-G8, ALB, Albumin

1 Images

Sandwich ELISA - Monkey Albumin ELISA Kit (AB190533)

sELISA

Supplier Data

Sandwich ELISA - Monkey Albumin ELISA Kit (AB190533)

Representative standard curve using ab190533 Albumin Monkey ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum

Reacts with

Monkey

Assay type

Sandwich (quantitative)

Sensitivity

= 1.795 ng/mL

Range

12.5 - 400 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Monkey Albumin ELISA Kit ab190533 is a sandwich ELISA kit to measure Monkey Albumin in Serum, Plasma, and Urine with a sensitivity of 1.795 ng/mL.

How the assay works

Monkey Albumin ELISA Kit ab190533 uses a pair of antibodies each specific of different epitopes at the target of interest : capture antibody is coated on the surface of the multi-well plate to facilitate the immobilization of the antigen. The other antibody is conjugated and facilitates the detection of the antigen.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.

Monkey Albumin ELISA Kit ab190533 protocol summary:

1. Add samples and standards to wells. Incubate at room temperature.
2. Wash each well and add enzyme-antibody conjugate. Incubate at room temperature.
3. Add TMB Solution to each well. Incubate at room temperature.
4. Add Stop Solution to each well. Read immediately.

Albumin (Alb) is a polyfunctional protein contributing to homeostasis through mechanisms of hemodynamics, transport and nutrition. Albumin is found both intra and extravascularly in all mammals and many lower vertebrates. It is a molecule of about 67,000 daltons, synthesized by the liver. Normally only very trace amounts of albumin escape reabsorption by kidney glomeruli and is excreted into the urine. Many occult diseases can cause kidney damage which may result in excessive amounts of serum proteins, including albumin, to be excreted by the kidney and into the urine. This ELISA kit can be used to measure albumin in serum, tissue extracts and other biological fluids.

The calibrator used in this kit is of human origin. It is quantitated with purified Cynomolgus monkey albumin and behaves identically with it.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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Recovery

[ { "sample": "Serum", "range": null, "average": ">= 85" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab190533-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB190533-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB190533-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB190533-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB190533-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Albumin Monkey HRP Conjugate", "number":"AB190533-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Albumin Monkey Calibrator", "number":"AB190533-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Albumin Monkey Antibody coated microwells", "number":"AB190533-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.

Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.

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Product protocols

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).

See full target information ALB

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Monkey Albumin ELISA Kit

Sandwich ELISA - Monkey Albumin ELISA Kit (AB190533)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise