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AB314364

Monkey Cardiac Troponin I ELISA Kit - Extracellular

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Monkey Cardiac Troponin I ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Monkey Cardiac Troponin I with a sensitivity of 2.075 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
2 Images
Sandwich ELISA - Monkey Cardiac Troponin I ELISA Kit - Extracellular (AB314364)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Cardiac Troponin I ELISA Kit - Extracellular (AB314364)

Recombinant Cardiac Troponin I was spiked into the following monkey samples and diluted in a 2-fold dilution series in Sample Diluent 50BP. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and RPMI1640 cell culture media (FBS-free) 50%.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2)

Sandwich ELISA - Monkey Cardiac Troponin I ELISA Kit - Extracellular (AB314364)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Cardiac Troponin I ELISA Kit - Extracellular (AB314364)

Example of monkey Cardiac Troponin I standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture media

Reacts with

Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.075 ng/mL

Range

2.656 - 170 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monkey Cardiac Troponin I SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cardiac Troponin I protein in Serum, Cit Plasma, EDTA Plasma, Cell culture media. Quantitate Monkey Cardiac Troponin I with 2.075 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
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Recovery

[ { "sample": "Serum", "range": "81 - 96 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "86 - 94 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "90 - 94 %", "average": "= 92" }, { "sample": "Cell culture media", "range": "103 - 114 %", "average": "= 109" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information TNNT1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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