Monkey Cardiac Troponin I ELISA Kit - Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Monkey Cardiac Troponin I - Extracellular in Serum, Citrate plasma, EDTA Plasma, Cell culture media samples.
Colorimetric
Serum, Citrate plasma, EDTA Plasma, Cell culture media
Sandwich (quantitative)
Monkey
2.656 - 170 ng/mL
1h 30m
= 2.075 ng/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Monkey Cardiac Troponin I ELISA Kit - Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Monkey Cardiac Troponin I - Extracellular in Serum, Citrate plasma, EDTA Plasma, Cell culture media samples.
Colorimetric
Serum, Citrate plasma, EDTA Plasma, Cell culture media
Sandwich (quantitative)
Monkey
2.656 - 170 ng/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 2.075 ng/mL
88%
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 5.6 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3.2 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 88 | Range 81 - 96 % |
Sample type Citrate plasma | Average % = 89 | Range 86 - 94 % |
Sample type EDTA Plasma | Average % = 92 | Range 90 - 94 % |
Sample type Cell culture media | Average % = 109 | Range 103 - 114 % |
Blue Ice
+4°C
+4°C
+4°C
Monkey Cardiac Troponin I SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cardiac Troponin I protein in Serum, Cit Plasma, EDTA Plasma, Cell culture media. Quantitate Monkey Cardiac Troponin I with 2.075 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Recombinant Cardiac Troponin I was spiked into the following monkey samples and diluted in a 2-fold dilution series in Sample Diluent 50BP. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and RPMI1640 cell culture media (FBS-free) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2)
Example of monkey Cardiac Troponin I standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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