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Monkey Cardiac Troponin I ELISA Kit - Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Monkey Cardiac Troponin I - Extracellular in Serum, Citrate plasma, EDTA Plasma, Cell culture media samples.

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Images

Sandwich ELISA - Monkey Cardiac Troponin I ELISA Kit - Extracellular (AB314364), expandable thumbnail
  • Sandwich ELISA - Monkey Cardiac Troponin I ELISA Kit - Extracellular (AB314364), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture media

Assay type

Sandwich (quantitative)

Reactive species

Monkey

Range

2.656 - 170 ng/mL

Assay time

1h 30m

Sensitivity

= 2.075 ng/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

1 x 96 Tests
Components
10X Monkey Cardiac Troponin I Capture Antibody
1 x 600 µL
10X Monkey Cardiac Troponin I Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent CPI2
1 x 6 mL
Monkey Cardiac Troponin I Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent 50BP
1 x 20 mL
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Monkey Cardiac Troponin I ELISA Kit - Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Monkey Cardiac Troponin I - Extracellular in Serum, Citrate plasma, EDTA Plasma, Cell culture media samples.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture media

Assay type

Sandwich (quantitative)

Reactive species

Monkey

Range

2.656 - 170 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 2.075 ng/mL

General recovery

88%

Precision

Intra assay

Sample

Serum

n

8

mean

-

SD

-

C.V.

5.6

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

3.2

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 88

Range

81 - 96 %

Sample type

Citrate plasma

Average %

= 89

Range

86 - 94 %

Sample type

EDTA Plasma

Average %

= 92

Range

90 - 94 %

Sample type

Cell culture media

Average %

= 109

Range

103 - 114 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Monkey Cardiac Troponin I SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cardiac Troponin I protein in Serum, Cit Plasma, EDTA Plasma, Cell culture media. Quantitate Monkey Cardiac Troponin I with 2.075 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

        -Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Monkey Cardiac Troponin I ELISA Kit - Extracellular (ab314364), expandable thumbnail

    Sandwich ELISA - Monkey Cardiac Troponin I ELISA Kit - Extracellular (ab314364)

    Recombinant Cardiac Troponin I was spiked into the following monkey samples and diluted in a 2-fold dilution series in Sample Diluent 50BP. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and RPMI1640 cell culture media (FBS-free) 50%.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2)

  • Sandwich ELISA - Monkey Cardiac Troponin I ELISA Kit - Extracellular (ab314364), expandable thumbnail

    Sandwich ELISA - Monkey Cardiac Troponin I ELISA Kit - Extracellular (ab314364)

    Example of monkey Cardiac Troponin I standard curve. Background-subtracted data values (mean +/- SD) are graphed. 

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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