Monkey CCL4 ELISA Kit
- Recombinant
- SimpleStep
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Monkey CCL4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey CCL4 with a sensitivity of 1.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
MIP1B, SCYA4, CCL4, C-C motif chemokine 4, Macrophage inflammatory protein 1-beta, Small-inducible cytokine A4, MIP-1-beta
- sELISA
Unknown
Sandwich ELISA - Monkey CCL4 ELISA Kit (AB242229)
Example of monkey CCL4 standard curve in 1X Cell Extraction Buffer PTR. The CCL4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Unknown
Sandwich ELISA - Monkey CCL4 ELISA Kit (AB242229)
Example of comparison of human and non-human primate CCL4. Standard Curve comparison between human and non-human primate CCL4.
- sELISA
Unknown
Sandwich ELISA - Monkey CCL4 ELISA Kit (AB242229)
Comparison of rhesus macaque and cynomolgus monkey serum, plasma, and PMA + Ionomycin stimulated PBMC cell culture supernatants (24 hours). The concentrations of CCL4 were measured in duplicates, interpolated from the CCL4 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values in neat samples are plotted (mean +/- SD, n=2).
- sELISA
Unknown
Sandwich ELISA - Monkey CCL4 ELISA Kit (AB242229)
Interpolated concentrations of native CCL4 in rhesus macaque serum and plasma samples. The concentrations of CCL4 were measured in duplicates, interpolated from the CCL4 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum neat, plasma (citrate) neat, plasma (heparin) neat, and plasma (EDTA) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL4 concentration was determined to be 140.2 pg/mL in neat serum, 101.6 pg/mL in neat plasma (citrate), 55.1 in neat plasma (heparin), and 109.5 pg/mL in neat plasma (EDTA).
- sELISA
Unknown
Sandwich ELISA - Monkey CCL4 ELISA Kit (AB242229)
Interpolated concentrations of native CCL4 in rhesus macaque stimulated peripheral blood mononuclear cell (PBMC) culture supernatant samples.
Rhesus macaque PBMCs were cultured in the presence or absence of 50 ng/mL PMA and 1 μg/mL Ionomycin for 24 hours. The concentrations of CCL4 were measured in duplicates, interpolated from the CCL4 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulate PBMC supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL4 concentration was determined to be 35.89 ng/mL in neat stimulated rhesus macaque PBMC cell culture supernatant and undetectable in unstimulated PBMC cell culture supernatant and naive cell culture media (not shown).
Reactivity data
Product details
Monkey CCL4 ELISA Kit (ab242229) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL4 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey CCL4 with 1.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CCL4 (MIP-1 beta) is a monokine with inflammatory and chemokinetic properties. CCL4 binds to CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form CCL4 retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T cells. This form of CCL4 is also a ligand for CCR1 and CCR2 isoform B. Structurally, CCL4 forms a heterodimer with MIP-1 alpha and is secreted from peripheral blood lymphocytes.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CCL4 is critical in immune system modulation. It coordinates leukocyte activation and trafficking contributing to inflammation and immune surveillance. It does not function in isolation but rather interacts with other chemokines and receptors. CCL4 alongside its sibling chemokines forms a dynamic network that ensures a balanced immune response. It connects to receptors such as CCR5 playing substantial roles in its activities.
Pathways
CCL4 participates in essential signaling cascades that mediate immune responses. It is notably part of the chemokine signaling pathway which is instrumental in directing cell movement. CCL4 also engages with proteins like CCR5 in the inflammatory and immune response pathways. This interaction enhances communication between cells required for effective pathogen defense.
Product protocols
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Target data
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