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AB324513

Monkey CD8 alpha ELISA Kit - Extracellular

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Monkey CD8 alpha ELISA Kit - Extracellular is a Sandwich ELISA for the measurement of Monkey CD8 alpha - Extracellular in Monkey in Biofluids, Other samples samples.
3 Images
Sandwich ELISA - Monkey CD8 alpha ELISA Kit - Extracellular (AB324513)
  • sELISA

Lab

Sandwich ELISA - Monkey CD8 alpha ELISA Kit - Extracellular (AB324513)

Example of monkey CD8 alpha standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Monkey CD8 alpha ELISA Kit - Extracellular (AB324513)
  • sELISA

Lab

Sandwich ELISA - Monkey CD8 alpha ELISA Kit - Extracellular (AB324513)

Example of monkey CD8 alpha standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Monkey CD8 alpha ELISA Kit - Extracellular (AB324513)
  • sELISA

Lab

Sandwich ELISA - Monkey CD8 alpha ELISA Kit - Extracellular (AB324513)

Interpolated concentration of native CD8 alpha was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 50%, plasma (citrate) 25%, plasma (EDTA) 50%, and plasma (heparin) 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Supernatant

Reacts with

Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.367 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Monkey CD8 alpha ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Monkey CD8 alpha with a sensitivity of 4.367pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4" } ]

Recovery

[ { "sample": "Serum", "range": "92 - 104 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "86 - 93 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "86 - 92 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "85 - 85 %", "average": "= 85" }, { "sample": "Cell culture supernatant", "range": "104 - 115 %", "average": "= 111" } ]

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Product protocols

websiteProtocolBooklet
en

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