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AB260062

Monkey CRP ELISA Kit (C-Reactive protein)

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(2 Publications)

Monkey CRP ELISA Kit (C-Reactive protein) is a single-wash 90-min Simplestep used to quantify Monkey CRP (C-Reactive protein) with a sensitivity of 9.14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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ELISA - Monkey CRP ELISA Kit (C-Reactive protein) (AB260062)
  • ELISA

Supplier Data

ELISA - Monkey CRP ELISA Kit (C-Reactive protein) (AB260062)
Sandwich ELISA - Monkey CRP ELISA Kit (C-Reactive protein) (AB260062)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey CRP ELISA Kit (C-Reactive protein) (AB260062)

Interpolated concentrations of CRP in rhesus macaque cerebrospinal fluid.

Interpolated concentration of native CRP was measured in duplicate at different sample concentrations. Undiluted samples are 3.13% rhesus macaque cerebrospinal fluid. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Monkey CRP ELISA Kit (C-Reactive protein) (AB260062)
  • sELISA

Lab

Sandwich ELISA - Monkey CRP ELISA Kit (C-Reactive protein) (AB260062)

Example of monkey CRP standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Monkey CRP ELISA Kit (C-Reactive protein) (AB260062)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey CRP ELISA Kit (C-Reactive protein) (AB260062)

Interpolated concentrations of native CRP in monkey serum and plasma samples.

The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 500, plasma (citrate) 1 : 500, plasma (EDTA) 1 : 2000 and plasma (heparin) 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 824.7 ng/mL in serum, 743.0 ng/mL in plasma (citrate) and 2689.5 ng/mL in plasma (EDTA), and 826.5 ng/mL in plasma (heparin).

Sandwich ELISA - Monkey CRP ELISA Kit (C-Reactive protein) (AB260062)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey CRP ELISA Kit (C-Reactive protein) (AB260062)

Example of monkey CRP standard curve in Sample Diluent NS.

The CRP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Monkey

Assay type

Sandwich (quantitative)

Sensitivity

= 9.14 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monkey CRP ELISA Kit (C-Reactive protein) ab260062 is a rapid single-wash 90-min Sandwich ELISA to measure Monkey CRP (C-Reactive protein)in cell culture media, CSF, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 9.14 pg/mL.

How the assay works

Monkey CRP (C-Reactive protein)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Monkey CRP ELISA Kit (C-Reactive protein) ab260062 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Monkey CRP ELISA Kit (C-Reactive protein) only requires a maximum of 12.5µL sample

C-reactive protein (CRP) displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. CRP can interact with DNA and histones and it may scavenge nuclear material released from damaged circulating cells. CRP is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. CRP binds 2 calcium ions per subunit. The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1 and IL6//interleukin-6. This kit requires the use of acid treatment for the disassociation of CRP.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]
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Recovery

[ { "sample": "Cerebral Spinal Fluid", "range": "99 - 110 %", "average": "= 104" }, { "sample": "Serum", "range": "110 - 119 %", "average": "= 115" }, { "sample": "EDTA Plasma", "range": "111 - 117 %", "average": "= 115" }, { "sample": "Cell culture media", "range": "97 - 103 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "112 - 115 %", "average": "= 113" }, { "sample": "Citrate plasma", "range": "113 - 117 %", "average": "= 115" } ]
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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab260062-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB260062-CMP04" }, { "size":"1 x 600 µL", "name":"10X Monkey CRP (C-Reactive Protein) Capture Antibody", "number":"AB260062-CMP07" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB260062-CMP10" }, { "size":"1 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB260062-CMP02" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB260062-CMP06" }, { "size":"2 x 1 Vial", "name":"Monkey CRP (C-Reactive Protein) Lyophilized Recombinant Protein", "number":"AB260062-CMP01" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB260062-CMP05" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB260062-CMP09" }, { "size":"1 x 600 µL", "name":"10X Monkey CRP (C-Reactive Protein) Detector Antibody", "number":"AB260062-CMP08" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB260062-CMP11" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab260062-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB260062-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB260062-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB260062-CMP05" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB260062-CMP06" }, { "size":"1 x 6000 µL", "name":"10X Monkey CRP (C-Reactive Protein) Capture Antibody", "number":"AB260062-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB260062-CMP02" }, { "size":"2 x 10 Vial", "name":"Monkey CRP (C-Reactive Protein) Lyophilized Recombinant Protein", "number":"AB260062-CMP01" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB260062-CMP03" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB260062-CMP09" }, { "size":"1 x 6000 µL", "name":"10X Monkey CRP (C-Reactive Protein) Detector Antibody", "number":"AB260062-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab260062-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB260062-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey CRP (C-Reactive Protein) Capture Antibody", "number":"AB260062-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB260062-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Monkey CRP (C-Reactive Protein) Lyophilized Recombinant Protein", "number":"AB260062-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB260062-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB260062-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB260062-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey CRP (C-Reactive Protein) Detector Antibody", "number":"AB260062-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB260062-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB260062-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.
Biological function summary

CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.

Pathways

CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.

Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.
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Product protocols

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Target data

See full target information CRP
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Viruses 17: PubMed40284993

2025

Inactivated Viral Vaccine BBV87 Protects Against Chikungunya Virus Challenge in a Non-Human Primate Model.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah L Kempster,Deborah Ferguson,Claire Ham,Joanna Hall,Adrian Jenkins,Elaine Giles,Simon L Priestnall,Alejandro Suarez-Bonnet,Pierre Roques,Roger Le Grand,Sumathy Kandaswamy,Sushant Sahastrabuddhe,Libia Milena Hernandez,Sunee Chuasuwan,Hyeon Seon Ahn,Deok Ryun Kim,Anh Wartel,Raphaël M Zellweger,Neil Berry,Neil Almond

Viruses 14: PubMed35891560

2022

Histopathological and Immunological Findings in the Common Marmoset Following Exposure to Aerosolized SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel E Ireland,Carwyn D Davies,Emma Keyser,James S F Findlay,Lin Eastaugh,Thomas R Laws,Francisco J Salguero,Laura Hunter,Michelle Nelson
View all publications
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