Monkey Cystatin C ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Monkey Cystatin C in Monkey in Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Monkey
- Range
- 0.125 - 4 ng/mL
- Sensitivity
- = 0.3303 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Alternative names
Cystatin-C, Cystatin-3, CST3
What's included?
Recommended products
Monkey Cystatin C ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Monkey Cystatin C in Monkey in Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Monkey
- Range
- 0.125 - 4 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- = 0.3303 ng/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 0 | C.V. < 10 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 0 | C.V. < 10 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % > 85 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Cystatin C Monkey ELISA Kit (ab190542) is for the measurement of Cystatin C in monkey serum and plasma.
In this assay the Cystatin C present in samples reacts with the anti-Cystatin C antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-Cystatin C, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Cystatin C in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Cystatin C in the test sample. The quantity of Cystatin C in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Cystatin C is a small cysteine proteinase inhibitor present in body fluids. Studies have shown Cystatin C levels to be directly correlated with the glomular filtration rate.
The calibrator used in this kit is of human origin.
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1 product image
Sandwich ELISA - Monkey Cystatin C ELISA Kit (ab190542)
Representative standard curve using Cystatin Monkey ELISA kit (ab190542)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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