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AB190542

Monkey Cystatin C ELISA Kit

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Monkey Cystatin C ELISA Kit is a Sandwich ELISA for the measurement of Monkey Cystatin C in Monkey in Biofluids samples.

View Alternative Names

Cystatin-C, Cystatin-3, CST3

1 Images
Sandwich ELISA - Monkey Cystatin C ELISA Kit (AB190542)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Cystatin C ELISA Kit (AB190542)

Representative standard curve using Cystatin Monkey ELISA kit (ab190542)

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.3303 ng/mL

Range

0.125 - 4 ng/mL

Assay Platform

Microplate

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Complementary Products

Primary Antibodies

AB109508

Anti-Cystatin C antibody [EPR4413]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (AB109508)

  • 5
  • 2
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Cystatin C Monkey ELISA Kit (ab190542) is for the measurement of Cystatin C in monkey serum and plasma.

In this assay the Cystatin C present in samples reacts with the anti-Cystatin C antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-Cystatin C, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Cystatin C in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Cystatin C in the test sample. The quantity of Cystatin C in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Cystatin C is a small cysteine proteinase inhibitor present in body fluids. Studies have shown Cystatin C levels to be directly correlated with the glomular filtration rate.

The calibrator used in this kit is of human origin.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab190542-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB190542-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB190542-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Cystatin C Monkey Detection Antibody", "number":"AB190542-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Cystatin C Monkey HRP Streptavidin Conjugate", "number":"AB190542-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Cystatin C Monkey Calibrator (lyophilized)", "number":"AB190542-CMP02", "productcode":"" }, { "size":"1 x 96 Test", "name":"Cystatin C Monkey Antibody coated microwells", "number":"AB190542-CMP01", "productcode":"" }, { "size":"1 x 60 mL", "name":"1X Diluent Concentrate", "number":"AB190542-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB190542-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
See full target information CST3
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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