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AB316888

Monkey G-CSF ELISA Kit

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Monkey G-CSF ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey G-CSF with a sensitivity of 5.161 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - Monkey G-CSF ELISA Kit (AB316888)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey G-CSF ELISA Kit (AB316888)

Example of monkey G-CSF standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Monkey G-CSF ELISA Kit (AB316888)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey G-CSF ELISA Kit (AB316888)

Interpolated concentration of native G-CSF was measured in duplicate at different sample concentrations. Undiluted samples are 100% unstimulated and stimulated PBMC cell culture supernatant (treated with 50 ng/mL PMA and 1 µg/mL Ionomycin for 24 hrs). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No signal was detected in unstimulated PBMC cell culture supernatant.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Urine, Cell culture supernatant

Reacts with

Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.161 pg/mL

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monkey G-CSF SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of G-CSF protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Urine, Cell culture supernatant. Quantitate Monkey G-CSF with 5.161 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]
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Recovery

[ { "sample": "Serum", "range": "99 - 100 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "91 - 97 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "90 - 94 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "88 - 93 %", "average": "= 91" }, { "sample": "Urine", "range": "102 - 104 %", "average": "= 103" }, { "sample": "Cell culture supernatant", "range": "102 - 107 %", "average": "= 105" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information CSF3
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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