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AB269556

Monkey Galectin-3 ELISA Kit (LGALS3)

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Monkey Galectin-3 ELISA Kit (LGALS3) is a single-wash 90-min Simplestep used to quantify Monkey Galectin-3 (LGALS3) with a sensitivity of 115 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - Monkey Galectin-3 ELISA Kit (LGALS3) (AB269556)
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Supplier Data

Sandwich ELISA - Monkey Galectin-3 ELISA Kit (LGALS3) (AB269556)

Interpolated concentrations of native Galectin-3 in Rhesus Macaque monkey serum and plasma samples.

The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 4%, plasma (citrate) 4%, plasma (EDTA) 4% and plasma (heparin) 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 93 ng/mL in neat serum, 111 ng/mL in neat plasma (citrate), 93 ng/mL in neat plasma (EDTA) and 88 ng/mL in neat plasma (heparin).

Sandwich ELISA - Monkey Galectin-3 ELISA Kit (LGALS3) (AB269556)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Galectin-3 ELISA Kit (LGALS3) (AB269556)

Interpolated concentrations of native Galectin-3 in Rhesus Macaque monkey PBMC cell culture supernatant cultured for 24 hours.

The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC supernatant 57%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 7.5 ng/mL in PBMC supernatant.

Sandwich ELISA - Monkey Galectin-3 ELISA Kit (LGALS3) (AB269556)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Galectin-3 ELISA Kit (LGALS3) (AB269556)

Example of monkey Galectin-3 standard curve in Sample Diluent NS.

The Galectin-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 115 pg/mL

Range

208.9 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monkey Galectin-3 ELISA Kit (LGALS3) (ab269556) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin-3 (LGALS3) protein in edta plasma, hep plasma, serum, cell culture media, cit plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey Galectin-3 (LGALS3) with 115 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Galectin-3 (Gal-3, Galactoside-binding protein, Mac-2 antigen) is a member of the galectin family of carbohydrate-binding proteins. Galectin-3 is active intracellularly and extracellularly. It is widely expressed, including by fibroblasts and macrophages. Intracellularly, Galectin-3 can function in the nucleus as a pre-mRNA splicing factor and in the cytoplasm it has roles in endocytosis, exocytosis and apoptosis. Extracellularly, Galectin-3 is often pro-inflammatory and is involved in innate immunity. Galectin-3 can be monomeric or form higher order homo and hetero oligomers.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 90 %", "average": "= 87" }, { "sample": "Serum", "range": "100 - 110 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "102 - 110 %", "average": "= 106" }, { "sample": "Cell culture media", "range": "81 - 85 %", "average": "= 83" }, { "sample": "Heparin Plasma", "range": "108 - 110 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "116 - 118 %", "average": "= 117" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.
Biological function summary

Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.

Pathways

Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like β-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.

Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-β. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.
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Product protocols

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Target data

See full target information Galectin
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websiteProtocolBooklet
en
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Product promise

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