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AB252361

Monkey GM-CSF ELISA Kit

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Monkey GM-CSF ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey GM-CSF with a sensitivity of 8.45 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Granulocyte-macrophage colony-stimulating factor, Colony Stimulating Factor 2, Pluripoietin-a

3 Images
Sandwich ELISA - Monkey GM-CSF ELISA Kit (AB252361)
  • sELISA

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Sandwich ELISA - Monkey GM-CSF ELISA Kit (AB252361)

Example of monkey GM-CSF standard curve in Sample Diluent NS.

The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Monkey GM-CSF ELISA Kit (AB252361)
  • sELISA

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Sandwich ELISA - Monkey GM-CSF ELISA Kit (AB252361)

Interpolated concentrations of spike GM-CSF in Rhesus macaque serum, plasma and cell culture media samples

Sandwich ELISA - Monkey GM-CSF ELISA Kit (AB252361)
  • sELISA

Unknown

Sandwich ELISA - Monkey GM-CSF ELISA Kit (AB252361)

Interpolated concentrations of native GM-CSF in Rhesus macaque PBMC (PMA-stimulated) cell culture supernatant.

The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted sample is as follows : PBMC (PMA-stimulated) cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 3726.5 pg/mL in PBMC (PMA-stimulated) cell culture supernatant. GM-CSF was undetectable in unstimulated PBMC cell culture supernatant.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Monkey

Assay type

Sandwich (quantitative)

Sensitivity

= 8.45 pg/mL

Range

15.63 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monkey GM-CSF ELISA Kit (ab252361) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GM-CSF protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey GM-CSF with 8.45 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]
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Recovery

[ { "sample": "Serum", "range": "93 - 97 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "93 - 97 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "95 - 98 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "95 - 98 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "91 - 96 %", "average": "= 93" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab252361-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB252361-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB252361-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB252361-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB252361-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB252361-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey GM-CSF Capture Antibody", "number":"AB252361-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB252361-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Monkey GM-CSF Lyophilized Recombinant Protein", "number":"AB252361-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB252361-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey GM-CSF Detector Antibody", "number":"AB252361-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD116 also known as the CSF2RA (colony-stimulating factor 2 receptor alpha subunit) is a protein that plays an important role in cytokine receptor signaling. It has a mass of approximately 43 kDa. This receptor is widely expressed on the surface of hematopoietic cells including monocytes granulocytes and some T-lymphocytes. Furthermore CD116 presents important interactions with cellular functioning by being part of a heterodimeric receptor complex. It couples with the beta subunit (CSF2RB) together mediating effects of granulocyte-macrophage colony-stimulating factor (GM-CSF).
Biological function summary

The CSF2RA is integral in facilitating cellular communication. It belongs to the cytokine receptor family specifically binding the GM-CSF. This molecule triggers signaling cascades that are essential for the proliferation differentiation and survival of various blood cells. When GM-CSF binds to the receptor complex made of CD116 and CSF2RB it activates downstream pathways influencing these critical processes thereby ensuring proper bone marrow function and immune cell activity.

Pathways

CD116 participates in significant signal transduction cascades. It is involved in the JAK-STAT pathway which plays an important role in regulating immune responses and hematopoiesis. The engagement of GM-CSF with CD116 triggers the activation of JAK kinases followed by STAT protein phosphorylation. Another pathway linked with CD116 is the MAPK pathway which further mediates downstream signals that modulate cellular growth and differentiation integrating input from various activating signals.

Abnormalities in CD116 expression and function associate with several health conditions. One example is chronic myelomonocytic leukemia (CMML) where deregulated signaling through the GM-CSF receptor impacts cell proliferation and differentiation. Aberrant CD116 activity also ties into inflammation and autoimmune disorders such as rheumatoid arthritis where overactive signaling can worsen inflammatory responses. Proteins like GM-CSF and its beta receptor subunit CSF2RB accompany these disorders by contributing to the pathway dysregulation observed in these disease states.
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Product protocols

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Target data

See full target information CSF2
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websiteProtocolBooklet
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