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AB322434

Monkey H-FABP ELISA Kit

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Monkey H-FABP ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey H-FABP with a sensitivity of 1.902 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
5 Images
Sandwich ELISA - Monkey H-FABP ELISA Kit (AB322434)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey H-FABP ELISA Kit (AB322434)

Interpolated concentration of native H-FABP was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Rhesus macaque serum 1%, plasma citrate 2%, plasma EDTA 2%, and plasma heparin 1%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Monkey H-FABP ELISA Kit (AB322434)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey H-FABP ELISA Kit (AB322434)

Interpolated concentration of native H-FABP was measured in duplicate at different sample concentrations. Undiluted sample is 25% monkey cerebral spinal fluid (CSF). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Monkey H-FABP ELISA Kit (AB322434)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey H-FABP ELISA Kit (AB322434)

Example of monkey H-FABP standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Monkey H-FABP ELISA Kit (AB322434)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey H-FABP ELISA Kit (AB322434)

Example of monkey H-FABP standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Monkey H-FABP ELISA Kit (AB322434)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey H-FABP ELISA Kit (AB322434)

Interpolated concentration of native H-FABP was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1% monkey serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid

Reacts with

Monkey

Assay type

Sandwich (quantitative)

Sensitivity

= 1.902 pg/mL

Range

5.625 - 360 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monkey H-FABP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of H-FABP protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, and Cerebral Spinal Fluid. Quantitate Monkey H-FABP with 1.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" } ]
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Recovery

[ { "sample": "Serum", "range": "84 - 108 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "90 - 101 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "97 - 103 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "101 - 105 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "95 - 108 %", "average": "= 100" }, { "sample": "Cerebral Spinal Fluid", "range": "96 - 117 %", "average": "= 110" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information FABP3
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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