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AB270895

Monkey IFN gamma ELISA Kit

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Monkey IFN gamma ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IFN gamma with a sensitivity of 65 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)

View Alternative Names

Interferon gamma, IFN-gamma, IFNG

4 Images
ELISA - Monkey IFN gamma ELISA Kit (AB270895)
  • ELISA

Supplier Data

ELISA - Monkey IFN gamma ELISA Kit (AB270895)
Sandwich ELISA - Monkey IFN gamma ELISA Kit (AB270895)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey IFN gamma ELISA Kit (AB270895)

Interpolated concentrations of native IFN gamma in cynomolgus monkey PBMC cell culture supernatant samples treated with or without PMA (50 ng/mL) and Ionomycin (1 µg/mL) for 24 hours.

The concentrations of IFN gamma were measured in duplicates, interpolated from the IFN gamma standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC treated supernatant 10%, and PBMC untreated supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 25 ng/mL in neat PBMC treated supernatant and undetectable in PBMC untreated supernatant.

Sandwich ELISA - Monkey IFN gamma ELISA Kit (AB270895)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey IFN gamma ELISA Kit (AB270895)

Example of monkey IFN gamma standard curve in Sample Diluent 75BP.

The IFN gamma standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Monkey IFN gamma ELISA Kit (AB270895)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey IFN gamma ELISA Kit (AB270895)

Example of monkey IFN gamma standard curve in Sample Diluent NS.

Example of monkey IFN gamma standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Monkey, Rhesus monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 65 pg/mL

Range

0.16 - 10 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monkey IFN gamma ELISA Kit ab270895 is a rapid single-wash 90-min Sandwich ELISA to measure Monkey IFN gamma in cell culture supernatant, CSF, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 65 pg/mL.

How the assay works

Monkey IFN gamma SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Monkey IFN gamma ELISA Kit ab270895 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

IFN gamma (Interferon-gamma), is an important immunoregulatory cytokine in addition of having antiviral activity. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. IFN gamma is produced by lymphocytes activated by specific antigens or mitogens.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]
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Recovery

[ { "sample": "Cerebral Spinal Fluid", "range": "98 - 109 %", "average": "= 102" }, { "sample": "Cell culture supernatant", "range": "97 - 117 %", "average": "= 109" }, { "sample": "Serum", "range": "75 - 92 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "95 - 111 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "80 - 118 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "82 - 89 %", "average": "= 85" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-?) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-? has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-? binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-? is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naпve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-? is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-? also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-? is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-? are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-? and its interactions can be key in developing therapeutic approaches against these conditions.
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Product protocols

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Target data

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (By similarity). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
See full target information IFNG
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websiteProtocolBooklet
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Product promise

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