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AB119530

Monkey IL-10 ELISA Kit

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Monkey IL-10 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Monkey IL-10 in Monkey in Cell Culture Media, Biofluids samples.

View Alternative Names

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

1 Images
Sandwich ELISA - Monkey IL-10 ELISA Kit (AB119530)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey IL-10 ELISA Kit (AB119530)

Representative Standard Curve using ab119530.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Monkey

Assay type

Sandwich (quantitative)

Sensitivity

= 1.3 U/mL

Range

3.9 - 250 U/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-10 Monkey ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Monkey IL-10 in cell culture supernatant and serum (baboon, cynomolgus).

This assay employs an antibody specific for Monkey IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Monkey IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Standard is recombinant protein produced in E.coli.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 5" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119530-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"20X Assay Buffer Concentrate", "number":"AB119530-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB119530-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"IL-10 Standard lyophilized (500 U/mL upon reconstitution)", "number":"AB119530-CMP05", "productcode":"" }, { "size":"1 x 70 µL", "name":"Biotin-Conjugate anti-monkey IL-10 monoclonal antibody", "number":"AB119530-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Microplate coated with monoclonal antibody to IL-10 (12 x 8 wells)", "number":"AB119530-CMP04", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Films", "number":"AB119530-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB119530-CMP08", "productcode":"" }, { "size":"1 x 150 µL", "name":"Streptavidin-HRP", "number":"AB119530-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution (1M Phosphoric acid)", "number":"AB119530-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
See full target information IL10
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websiteProtocolBooklet
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