Monkey IL-11 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IL-11 with a sensitivity of 26.73 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- EDTA Plasma, Cell culture supernatant, Heparin Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Monkey
- Range
- 156.25 - 10000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 26.73 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation. Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling'.
Alternative names
Interleukin-11, IL-11, IL11
What's included?
Recommended products
Monkey IL-11 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IL-11 with a sensitivity of 26.73 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Key facts
- Detection method
- Colorimetric
- Sample types
- EDTA Plasma, Cell culture supernatant, Heparin Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Monkey
- Range
- 156.25 - 10000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 26.73 pg/mL
- General recovery
- 93%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Plasma | n 8 | C.V. 3.6 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Plasma | n 3 | C.V. 3.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type EDTA Plasma | Average % = 93 | Range 81 - 103 % |
Sample type Cell culture supernatant | Average % = 112 | Range 108 - 115 % |
Sample type Heparin Plasma | Average % = 99 | Range 94 - 106 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Monkey IL-11 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-11 protein in EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Monkey IL-11 with 26.73 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Monkey IL-11 ELISA Kit (ab317547)
Example of monkey IL-11 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Monkey IL-11 ELISA Kit (ab317547)
Example of monkey IL-11 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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