AB283537

Monkey IL-17A ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Monkey IL-17A ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IL-17A with a sensitivity of 12 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CTLA-8, Cytotoxic T lymphocyte associated protein 8, Cytotoxic T lymphocyte associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL17_HUMAN, Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8), Interleukin-17A, Interleukin17, OTTHUMP00000016597, OTTMUSP00000046003

2 Images

Sandwich ELISA - Monkey IL-17A ELISA Kit (AB283537)

sELISA

Supplier Data

Sandwich ELISA - Monkey IL-17A ELISA Kit (AB283537)

Example of monkey IL-17A standard curve in Sample Diluent NS.

The IL-17A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Monkey IL-17A ELISA Kit (AB283537)

Interpolated concentrations of native IL-17A in Rhesus macaque cell culture supernatant samples.

The concentrations of IL-17A were measured in duplicates, interpolated from the IL-17A standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated (50 ng/mL PMA, 1 μg/mL Ionomycin, 24 hours, 37°C) and unstimulated PBMC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17A concentration was undetectable in unstimulated PBMC supernatant and determined to be 18 ng/mL in stimulated PBMC supernatant.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant

Reacts with

Monkey

Assay type

Sandwich (quantitative)

Sensitivity

= 12 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Monkey IL-17A ELISA kit (ab283537) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Monkey IL-17A protein in human cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey IL-17A with 12 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IL-17A is a proinflammatory cytokine that is secreted by activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce proinflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 103 %", "average": "= 99" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283537-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB283537-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB283537-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB283537-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB283537-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB283537-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Monkey IL-17A Lyophilized Recombinant Protein", "number":"AB283537-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB283537-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey IL-17A Capture Antibody", "number":"AB283537-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB283537-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey IL-17A Detector Antibody", "number":"AB283537-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

See full target information IL17A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 10:e624 PubMed35634952

2022

Effects of Astragalus membranaceus on systemic lupus erythematosus in a mouse model of pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Qing Chen,Na Zhang,Cai-Xia Li,Hong-Xia Zhang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Monkey IL-17A ELISA Kit

Sandwich ELISA - Monkey IL-17A ELISA Kit (AB283537)

Sandwich ELISA - Monkey IL-17A ELISA Kit (AB283537)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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