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AB242234

Monkey IL-1beta ELISA Kit

4

(1 Review)

|

(4 Publications )

Monkey IL-1beta ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IL-1beta with a sensitivity of 15.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
2 Images
Sandwich ELISA - Monkey IL-1beta ELISA Kit (AB242234)
  • sELISA

Unknown

Sandwich ELISA - Monkey IL-1beta ELISA Kit (AB242234)

Interpolated concentrations of spike IL-1beta in Rhesus monkey serum, plasma, and cell culture media (RPMI 1640 containing 10% fetal bovine serum) samples. The concentrations of IL-1beta were measured in duplicates, interpolated from the IL-1beta standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (heparin) 25%, and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Monkey IL-1beta ELISA Kit (AB242234)
  • sELISA

Unknown

Sandwich ELISA - Monkey IL-1beta ELISA Kit (AB242234)

Example of monkey IL-1beta standard curve in Sample Diluent NS. The IL-1beta standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Monkey, Cynomolgus monkey, Rhesus monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 15.2 pg/mL

Range

28.1 - 1800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monkey IL-1beta ELISA Kit (ab242234) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-1beta protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey IL-1beta with 15.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Media", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Cell Media", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]
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Recovery

[ { "sample": "Serum", "range": "86 - 92 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "76 - 83 %", "average": "= 80" }, { "sample": "Heparin Plasma", "range": "75 - 79 %", "average": "= 77" }, { "sample": "Citrate plasma", "range": "83 - 86 %", "average": "= 85" }, { "sample": "Cell culture media", "range": "88 - 93 %", "average": "= 91" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab242234-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB242234-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB242234-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB242234-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB242234-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB242234-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB242234-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB242234-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey IL-1beta Capture Antibody", "number":"AB242234-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Monkey IL-1beta Lyophilized recombinant Protein", "number":"AB242234-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey IL-1beta Detector Antibody", "number":"AB242234-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
See full target information IL1B

Alternative Names

Interleukin-1 beta, IL-1 beta, IL1B
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Neuroreport 35:780-789 PubMed38935074

2024

Electroacupuncture improves the learning and memory abilities of rats with PSCI by attenuating the TLR4/NF-κB/NLRP3 signaling pathway on the hippocampal microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Bian,Chunxu Liu,Xiang Li,Xiaoye Qin,Feng Wang,Lina Xuan,Weimin Zhang

Brain and behavior 13:e3275 PubMed37837628

2023

Inhibitory effect and mechanism of Rosiglitazone on M1 type polarization of central microglia in intracerebral hemorrhage mice based on JNK/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chenglei Chao,Yinghui Li,Quan Li,Guofeng Wu

Annals of palliative medicine 10:5299-5309 PubMed34107698

2021

Intermittent fasting therapy promotes insulin sensitivity by inhibiting NLRP3 inflammasome in rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Bing-Jun Liang,Sheng-Rong Liao,Wei-Xuan Huang,Chao Huang,Hao Sheng Liu,Wei-Zeng Shen

Molecular medicine reports 19:3555-3563 PubMed30864712

2019

Estrogen receptor β suppresses inflammation and the progression of prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Long Xiao,Yaohui Luo,Rongfen Tai,Ningnan Zhang
View all publications
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websiteProtocolBooklet
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Product promise

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