Monkey IL-2 ELISA Kit
- Recombinant
- SimpleStep
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Monkey IL-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IL-2 with a sensitivity of 12 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
View Alternative Names
Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2
- sELISA
Supplier Data
Sandwich ELISA - Monkey IL-2 ELISA Kit (AB283555)
Interpolated concentrations of native IL-2 in Rhesus macaque cell culture supernatant samples.
The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated (50 ng/mL PMA, 1 μg/mL Ionomycin, 24hours, 37°C) and unstimulated PBMC supernatant 1 : 400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2 concentration was undetectable in unstimulated PBMC supernatant and determined to be 179 ng/mL in stimulated PBMC supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Monkey IL-2 ELISA Kit (AB283555)
Example of monkey IL-2 standard curve in Sample Diluent NS.
The IL-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Product details
Monkey IL-2 ELISA kit (ab283555) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Monkey IL-2 protein in monkey cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey IL-2 with 12 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical polypeptide. It is secreted by activated CD4+ and CD8+ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation, Natural Killer cells (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death. IL-2 modulates the expression of receptors for other cytokines and transcription factors, therefore regulating cytokine cascades that correlate with each of the Th differentiation states. IL-2 is both an immune stimulator and immune suppressor cytokine, which efficiently controls the immune system to deal with autoimmunity and adaptive immune response.
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