AB242230

Monkey IL-4 ELISA Kit

Recombinant
SimpleStep
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(2 Publications)

Monkey IL-4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IL-4 with a sensitivity of 2.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interleukin-4, IL-4, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1, IL4

4 Images

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)

sELISA

Unknown

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)

Example of monkey IL-4 standard curve in Sample Diluent 75BP. The IL-4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)

Comparison of native IL-4 in rhesus and cynomolgus stimulated peripheral blood mononuclear cell (PBMC) supernatant samples.

PBMCs were cultured in the presence or absence of 50 ng/mL PMA and 1 μg/mL Ionomycin for 24 hours. The concentrations of IL-4 were measured in duplicates, interpolated from the IL-4 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-4 concentration was determined to be 95.34 pg/mL in neat stimulated rhesus PBMC supernatant and 47.30 pg/mL in neat stimulated cynomolgus PBMC supernatant. Values for unstimulated supernatants were undetectable in both rhesus and cynomolgus (not shown).

sELISA

Unknown

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)

Interpolated concentrations of native IL-4 in rhesus macaque stimulated peripheral blood mononuclear cell (PBMC) Supernatant samples.

Rhesus macaque PBMCs were cultured in the presence or absence of 50 ng/mL PMA and 1 μg/mL Ionomycin for 24 hours. The concentrations of IL-4 were measured in duplicates, interpolated from the IL-4 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-4 concentration was determined to be 95.34 pg/mL in neat stimulated PBMC supernatant, and undetectable in unstimulated PBMC supernatant and naive cell culture media (not shown).

sELISA

Unknown

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)

Example of monkey IL-4 standard curve in Sample Diluent NS. The IL-4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Monkey, Cynomolgus monkey, Rhesus monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.2 pg/mL

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Monkey IL-4 ELISA Kit (ab242230) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-4 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey IL-4 with 2.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Protein", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" }, { "reproducibilityType": "Intra", "sample": "Protein", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "115 - 133 %", "average": "= 121" }, { "sample": "Serum", "range": "75 - 81 %", "average": "= 79" }, { "sample": "EDTA Plasma", "range": "94 - 98 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "108 - 116 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "85 - 90 %", "average": "= 87" }, { "sample": "Cell culture media", "range": "103 - 104 %", "average": "= 104" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab242230-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB242230-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB242230-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB242230-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB242230-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB242230-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB242230-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BP", "number":"AB242230-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB242230-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey IL-4 Capture Antibody", "number":"AB242230-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Monkey IL-4 Lyophilized recombinant Protein", "number":"AB242230-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey IL-4 Detector Antibody", "number":"AB242230-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.

See full target information IL4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 30:59 PubMed38745316

2024

Polyphyllin I alleviates neuroinflammation after cerebral ischemia-reperfusion injury via facilitating autophagy-mediated M2 microglial polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyang Kang,Qiuling Sang,Dingxi Liu,Libo Wang,Jia Li,Xiaoyang Liu

International archives of allergy and immunology 183:375-388 PubMed34942624

2021

Mechanism of miR-181a-5p in Regulatory T/T-Helper 17 Immune Imbalance and Asthma Development in Mice with Allergic Rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoyun He,Yujuan Chen,Xiaoer Chen,Binfan Yuan

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Monkey IL-4 ELISA Kit

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Assay Kits

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

Product promise